[Histonet] (no subject)

[b003046 <@t> nf.au.dk]

Hi, im trying to get the DAKO polyclonal rabbit anti-human c-kit (CD117) to work
in rats in paraffin. I have tryed using antigen retrieval in microwave and down
to 1:50 dilution. Also I have tryed the C-19 c-kit from Santa Cruz and used
both on paraffin and frozen tissue. It just doesnt want to work! Can anybody
help?

Thanks,
Mette K. Hagensen
Department of Cardiology B, Research Unit
Aarhus University Hospital, Skejby Hospital
Brendstrupgaardsvej 100
8200 Aarhus N
Denmark

Phone:         +45 89496237
Fax:        +45 89496009
Mail:        b003046 <@t> nf.au.dk