[Histonet] Immunoflouresence Questions

Rene J Buesa rjbuesa <@t> yahoo.com
Thu Oct 13 14:28:40 CDT 2005

You are right, immunofluorescence procedures will fade, and fast; that is the reason behind taking photographs of the results.
You may keep the slides in a refrigerator and will be able to register immunofluorescence again, but WEAKER, the next day.
How long will this fluorescence will remain visible in refrigerated slides depends on the case, but cannot be predicted in general. When the pathologist that is scheduled to read our procedures is not available, we keep the slides for the next day, but that is all; no permanent solution.
There is no media that will retard fading (is something inherent to the FITC conjugated antibody and has nothing to do with the medium.
Probably your clinician "heard" something but evidently does not know the procedural details, or he may know something that neither you or I know.
Rene J.

Nita Searcy <NSEARCY <@t> swmail.sw.org> wrote:
I have a tech that is asking these questions regarding possible "new
procedures". We have a new clinician that has "new" ideas.

1. Does storing slides in refrigerator after staining aid in anyway? (I
had never seen this before ; always stored in dark @ room temperature).

2. IS there a mounting media that aids in permanence? 

3. To my knowledge, these slides are not permanent (no matter what you
do) but will inspectors really expect to see them- even in a faded
condition? (we take pictures of the kidneys)


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