[Histonet] RE: Histonet Digest, Vol 23, Issue 7
Sherif Girees
sgirees <@t> pathwaydx.com
Thu Oct 6 11:36:50 CDT 2005
To Patsy Ruegg, HT(ASCP)QIHC
You have to anesthetize the animal first so the lungs will not claps, then open the chest cavity, make a small incision in the left and the ventricle, using a plastic syringe very gently inject PBS Ph 7.4 till the blood clears, the introduce the fix. We used that method to perfuse feline and swine as well as mice and rats for LT and EM.
Sherif Girees,BS HT(ASCP)QIHC
Manager Molecular Pathology
Pathway Diagnostics
3003 Malibu Canyon
Malibu CA.90265
Tel:310-774-3569
Fax:310-774-3556
s.girees <@t> pathwaydx.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, October 06, 2005 7:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 23, Issue 7
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Today's Topics:
1. Fetal mouse samples (Patsy Ruegg)
2. Re: Fetal mouse samples (Rene J Buesa)
3. 20 micron sections (cynthia haynes)
4. lymphatics (Hawkins, Hal K.)
5. Pax2 (Patti Loykasek)
6. RE: Positions in Louisiana (D. Ford)
7. RE: lymphatics (Luck, Greg D.)
8. Nevada Society of Histotechnology (connie grubaugh)
9. Re: lymphatics (John Kiernan)
10. Re: lymphatics (anh2006 <@t> med.cornell.edu)
11. Fixation using Modified Bouins and NBF (Ahmed, T (Tanni))
12. RE: 20 micron sections (Lee & Peggy Wenk)
13. GFAP protocol for fluorescence (Stephen.Eyres <@t> sanofi-aventis.com)
14. staining under hoods (Sauer, Barb)
15. RE: staining under hoods (Weems, Joyce)
16. amyloid in FNA smears (Houston, Ronnie)
17. High Complexity Testing - IHC (danaspears <@t> frontiernet.net)
18. Metachromasia of mast cells in epoxy resin sections
(gillian.2.brown <@t> gsk.com)
19. Histology Journals (Prior, Andrew)
20. Re: 20 micron sections (Rene J Buesa)
21. Re: Histology Journals (Rene J Buesa)
22. Re: Nevada Society of Histotechnology (Rene J Buesa)
23. Job Openings In Dallas, Texas (Debbiejsiena <@t> aol.com)
24. CAP question (Kapoor, Sue)
25. Region II Meeting November 4th and 5th, 2005 (Pamela Marcum)
26. AW: [Histonet] lymphatics (Gudrun Lang)
----------------------------------------------------------------------
Message: 1
Date: Wed, 5 Oct 2005 14:37:50 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] Fetal mouse samples
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <200510052037.j95Kbf9O022163 <@t> chip.viawest.net>
Content-Type: text/plain; charset="us-ascii"
I am processing whole fetal mouse samples (these are pretty big, they have
skin developed) and the investigator wants to have sections so that both
lungs show up at the same time in the same section. Can someone recommend
to me a way to dissect these to open them up so that they will fix and
process well but still maintain the whole body architecture for sectioning.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/>
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------------------------------
Message: 2
Date: Wed, 5 Oct 2005 14:00:18 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Fetal mouse samples
To: Patsy Ruegg <pruegg <@t> ihctech.net>, histonet <@t> pathology.swmed.edu
Message-ID: <20051005210019.5811.qmail <@t> web61211.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Patsy:
Many years ago I had to do that. Of the three "classical" sectioning planes (transverse, sagittal and frontal) the one to use is the frontal. Rest the embryo flat on its back and cut it open starting at nose/mouth level all the way back to the vertebral column in a way that the back half of the embryo can be separated from the front half. Try to harden it first with formaldehyde (4-6 hours will be enough; you should also inject in the abdomen a little amount of NBF also).
Try to leave as much material in the front so you will get to cut to the lungs when trimming down the block. I recommend you also to process it enclosed in a cassette that keeps the structures in place, and to extend the times, not so much in the dehydrating as in the infiltrating steps.
Had you been using the dehydrating/infiltrating steps I used to use (ethyl alcohol/isopropyl alcohol/mineral oil) you would get a better infiltration.
Hope this will help.
Rene J.
Patsy Ruegg <pruegg <@t> ihctech.net> wrote:
I am processing whole fetal mouse samples (these are pretty big, they have
skin developed) and the investigator wants to have sections so that both
lungs show up at the same time in the same section. Can someone recommend
to me a way to dissect these to open them up so that they will fix and
process well but still maintain the whole body architecture for sectioning.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
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any of its contents, by any person other than the intended recipient may
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------------------------------
Message: 3
Date: Wed, 5 Oct 2005 14:14:29 -0700 (PDT)
From: cynthia haynes <naje1972 <@t> yahoo.com>
Subject: [Histonet] 20 micron sections
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051005211429.83499.qmail <@t> web33002.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello, everyone I am in need of information. I am
cutting brain(human)tissue at 6 microns and 20
microns. The 6 microns sections are perfect. The 20
microns sections are an nightmare(and I mean Elm
street nightmare). Can anyone suggest the best way to
cut these sections without getting wrinkles and folds.
I am at my wits end.
Thanks in advance
Cynthia Haynes H.T.
------------------------------
Message: 4
Date: Wed, 5 Oct 2005 16:15:48 -0500
From: "Hawkins, Hal K." <hhawkins <@t> UTMB.EDU>
Subject: [Histonet] lymphatics
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8D6F233E2A5D574B929F3944F3316FD006310469 <@t> EXCH2K3.utmb.edu>
Content-Type: text/plain; charset="us-ascii"
For a model of necrotizing enterocolitis in the mouse we need to
distinguish small veins from lymphatics in the gut. Could someone
remind me of any immunostains or lectin that will allow us to tell the
two apart?
Many thanks,
Hal Hawkins, UTMB Galveston
------------------------------
Message: 5
Date: Wed, 05 Oct 2005 14:53:42 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: [Histonet] Pax2
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <BF699976.A038%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="ISO-8859-1"
Hi all. I have a question about an antibody to Pax2. We have just begun to
try to work up this antibody. It is supposedly upregulated in renal cell
carcinoma, so it should be positive & it has been. From the reading I have
done it should be negative in normal adult kidney. Our current problem is
that we are seeing staining on normal adult kidney. Any help or ideas are
appreciated.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
This email message, including any attachments, is for the sole use of the
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prohibited. If you are not the intended recipient, please contact the
sender by e-mail and destroy all copies of the original message or you may
call PhenoPath Laboratories, Seattle, WA U.S.A at (206) 374-9000.
------------------------------
Message: 6
Date: Wed, 5 Oct 2005 17:21:44 -0500
From: "D. Ford" <dford <@t> deltapathology.com>
Subject: [Histonet] RE: Positions in Louisiana
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <NGEDJAFAGMONMNIJBJAHKEEBCAAA.dford <@t> deltapathology.com>
Content-Type: text/plain; charset="iso-8859-1"
Delta Pathology, in Shreveport Louisiana, currently looking for HT/HTLs. If
you have been displaced by the hurricanes and want to return to your home
state or if you are looking to relocate please contact us for more
information.
Darlene Ford, B.S. CT, HT (ASCP)
Technical Supervisor
2915 Missouri Avenue
Shreveport, LA 71109
318-621-8820 phone
318-671-5922 fax
dford <@t> deltapathology.com
------------------------------
Message: 7
Date: Wed, 5 Oct 2005 15:44:14 -0700
From: "Luck, Greg D." <LuckG <@t> empirehealth.org>
Subject: RE: [Histonet] lymphatics
To: "'Hawkins, Hal K.'" <hhawkins <@t> UTMB.EDU>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<EDAC013F7055E1438FF70C30C43A4BE203036EC8 <@t> exchange05.inhs.org>
Content-Type: text/plain
Hal,
Why don't you try the tools offered on www.immunoquery.com (this one is the
best but you need to get a username and password) or www.IHCWorld.com.
These are both excellent IHC resources.
Greg Luck, BS, HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Medical Center
800 W. 5th Ave
Spokane, WA 99204
Phone 509.473.7077
Fax 509.473.7133
luckg <@t> empirehealth.org
www.deaconessmedicalcenter.org
-----Original Message-----
From: Hawkins, Hal K. [mailto:hhawkins <@t> UTMB.EDU]
Sent: Wednesday, October 05, 2005 2:16 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] lymphatics
For a model of necrotizing enterocolitis in the mouse we need to distinguish
small veins from lymphatics in the gut. Could someone remind me of any
immunostains or lectin that will allow us to tell the two apart?
Many thanks,
Hal Hawkins, UTMB Galveston
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 06 Oct 2005 03:57:47 +0000
From: "connie grubaugh" <conniegrubaugh <@t> hotmail.com>
Subject: [Histonet] Nevada Society of Histotechnology
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY107-F234548B23021CA3F5FDBF4D6850 <@t> phx.gbl>
Content-Type: text/plain; format="flowed"
The Nevada Society of HIstotechnology will be having their next
seminar Oct. 28th and 29th at Sunrise Hospital Auditorium in Las Vegas
Nevada.
There will be a Friday evening get together with the vendors. Drinks
and snacks will be provided. This will start at 5:30 pm.
Saturday sign in at 7 am. Coffee and rolls will be provided by Fisher
Scientific.
Meeting starts at 8 am.
First meeting will be Pam Marcum -Is Your Tissue Processor Fighting
You? Fight Back. This will be 3 ceu"s.
There will be a luncheon provided by Sakura and Cardinal Health. A
business
meeting will also be held at this time.
Afternoon meetings.
1 pm- Sandra L. Cummings, Putting the bug in Histotechnology, A basic
understanding of Microorganisms.
To follow Marianne Mailhoit- Exposure control plan for Blood Borne
Pathogens.
There will be no charge for this meeting.
Hope to see everyone there.
Vegas is the best place to be on Halloween weekend.
Please email me with any questions, or call 702-396-4079 or
702-938-3619.
Connie Grubaugh
Nevada Society of Histotechnology
Striving for Excellence Despite the Odds.
------------------------------
Message: 9
Date: Thu, 06 Oct 2005 00:18:11 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] lymphatics
To: "Hawkins, Hal K." <hhawkins <@t> UTMB.EDU>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <4344A583.842FBFA <@t> uwo.ca>
Content-Type: text/plain; charset=us-ascii
Dear Hal Hawkins,
The endothelium of lymphatic vessels can be stained by
enzyme activity histochemistry. The enzyme is a
5-nucleotidase (belongs to the phosphatase family).
Ordinarily this type of method is carried out with frozen
sections of tissue that has been minimally fixed in
formaldehyde or glutaraldehyde, or with unfixed cryostat
sections that have been briefly fixed in cold acetone. The
enzyme in lymphatics may be fairly robust because its
activity can survive embedding in glycol methacrylate.
The references below may help. If you choose one of these
methods it will be important to consult the original
publication because there's more than one sort of
5-nucleotidase. Each ref is followed by brief notes that I
took when reading the papers. [An R in the acquisition
number means I've got a Reprint of the whole paper; an A
usually means I have a copy of the paper's Abstract. No
letter with the acquisition number usually means I've seen
the whole paper and took notes in the library.]
8194R. Kato,S; Yasunaga,A; Uchida,U (1991):
Enzyme-histochemical method for identification of lymphatic
capillaries. Lymphology 24, 125-129.
Glycol methacrylate-embedded sections stained for
5'-nucleotidase & alkaline phosphatase. 5-N-ase in lymph
capills; AlkP-ase in blood capills.
9239R. Nishida,S; Ohkuma,M (1993): Enzyme-histochemical
staining of dermal lymphatic capillaries by guanylate
cyclase. Lymphology 26, 195-199.
Enzyme histochemical staining distinguishes lymph from
blood capillaries. Says 5-nucleotidase & adenylate cyclase
do so too. (Used unfixed cryostat sections.)
9665R. Okada,E (1994): An improved enzyme-histochemical
method for identification of lymphatic capillaries on
paraffin sections. Lymphology 27, Suppl, 732-735.
Staining of blood & lymph capillaries. Enzyme
histochemistry for 5-nucleotidase in presence of levamisole
for lymph capills; alkaline phosphatase for blood capills.
11114A. Miura,M; Kato,S; von Ludinghausen,M (1998):
Lymphatic drainage of the cerebrospinal fluid from monkey
spinal meninges with special reference to the distribution
of the epidural lymphatics. Arch. Histol. Cytol. 61(3, Aug),
277-286.
5'-nucleotidase staining for lymphatics; alk phosphatase
for blood capillaries (Kato et al '91,'93). Also traced
carbon particles from cisterna magna. Lymphatics and carbon
found on surfaces of cervical & thoracic (most at brachial
plexus levels) roots; not lumbosacral. Epidural lymphatics
most developed on dorsal surface of lower cervical dura.
I hope this helps.
--
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan[AT]uwo.ca
http://publish.uwo.ca/~jkiernan/
http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
"Hawkins, Hal K." wrote:
>
>
> For a model of necrotizing enterocolitis in the mouse we need to
> distinguish small veins from lymphatics in the gut. Could someone
> remind me of any immunostains or lectin that will allow us to tell the
> two apart?
>
> Many thanks,
>
> Hal Hawkins, UTMB Galveston
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 06 Oct 2005 00:57:15 -0400 (EDT)
From: anh2006 <@t> med.cornell.edu
Subject: Re: [Histonet] lymphatics
To: John Kiernan <jkiernan <@t> uwo.ca>, histonet <@t> lists.utsouthwestern.edu
Message-ID: <48830.66.108.123.192.1128574635.squirrel <@t> webmail>
Content-Type: text/plain; charset=iso-8859-1
I read Dr. Kiernan's post with much interest. Has anyone on Histonet had
direct experience with these protocols? I would love to get some first
hand experience feedback before I try it out.
Thanks,
Andrea
> Dear Hal Hawkins,
>
> The endothelium of lymphatic vessels can be stained by
> enzyme activity histochemistry. The enzyme is a
> 5-nucleotidase (belongs to the phosphatase family).
> Ordinarily this type of method is carried out with frozen
> sections of tissue that has been minimally fixed in
> formaldehyde or glutaraldehyde, or with unfixed cryostat
> sections that have been briefly fixed in cold acetone. The
> enzyme in lymphatics may be fairly robust because its
> activity can survive embedding in glycol methacrylate.
>
> The references below may help. If you choose one of these
> methods it will be important to consult the original
> publication because there's more than one sort of
> 5-nucleotidase. Each ref is followed by brief notes that I
> took when reading the papers. [An R in the acquisition
> number means I've got a Reprint of the whole paper; an A
> usually means I have a copy of the paper's Abstract. No
> letter with the acquisition number usually means I've seen
> the whole paper and took notes in the library.]
>
> 8194R. Kato,S; Yasunaga,A; Uchida,U (1991):
> Enzyme-histochemical method for identification of lymphatic
> capillaries. Lymphology 24, 125-129.
> Glycol methacrylate-embedded sections stained for
> 5'-nucleotidase & alkaline phosphatase. 5-N-ase in lymph
> capills; AlkP-ase in blood capills.
>
> 9239R. Nishida,S; Ohkuma,M (1993): Enzyme-histochemical
> staining of dermal lymphatic capillaries by guanylate
> cyclase. Lymphology 26, 195-199.
> Enzyme histochemical staining distinguishes lymph from
> blood capillaries. Says 5-nucleotidase & adenylate cyclase
> do so too. (Used unfixed cryostat sections.)
>
> 9665R. Okada,E (1994): An improved enzyme-histochemical
> method for identification of lymphatic capillaries on
> paraffin sections. Lymphology 27, Suppl, 732-735.
> Staining of blood & lymph capillaries. Enzyme
> histochemistry for 5-nucleotidase in presence of levamisole
> for lymph capills; alkaline phosphatase for blood capills.
>
> 11114A. Miura,M; Kato,S; von Ludinghausen,M (1998):
> Lymphatic drainage of the cerebrospinal fluid from monkey
> spinal meninges with special reference to the distribution
> of the epidural lymphatics. Arch. Histol. Cytol. 61(3, Aug),
> 277-286.
> 5'-nucleotidase staining for lymphatics; alk phosphatase
> for blood capillaries (Kato et al '91,'93). Also traced
> carbon particles from cisterna magna. Lymphatics and carbon
> found on surfaces of cervical & thoracic (most at brachial
> plexus levels) roots; not lumbosacral. Epidural lymphatics
> most developed on dorsal surface of lower cervical dura.
>
> I hope this helps.
> --
> -------------------------------
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
> kiernan[AT]uwo.ca
> http://publish.uwo.ca/~jkiernan/
> http://instruct.uwo.ca/anatomy/530/index.htm
------------------------------
Message: 11
Date: Thu, 6 Oct 2005 09:05:33 +0100
From: "Ahmed, T \(Tanni\)" <Tanni.Ahmed <@t> intervet.com>
Subject: [Histonet] Fixation using Modified Bouins and NBF
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <09E7875E806DFB4BBECBADBF966BDBB6F6742B <@t> mksn79.d50.intra>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters,
I would like to know if fixation of avian bursa tissue with Modified
Bouins fixative over 10% Neutral Buffered Formalin has any advantagous
effect on immuno precipitation?
Thanks in advance,
Tanni
Tanni S Ahmed
Scientific Officer - Histopathology, R&D
Intervet UK Ltd.
Walton Manor,
Walton, Milton Keynes,
Buckinghamshire,
MK7 7AJ, UK.
Tel. +44(0)1908 685552/685543
Fax +44(0)1908 685614
E-mail: tanni.ahmed <@t> intervet.com
--------------------------------------
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Message: 12
Date: Thu, 6 Oct 2005 05:03:32 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] 20 micron sections
To: "'cynthia haynes'" <naje1972 <@t> yahoo.com>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.65.1128609992.20696.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Several suggestions:
- Cut slower (real slow; real, real slow)
- Cut the blocks at room temperature (not iced/chilled). Chilled is great
for getting thiner sections, warmer for thicker sections
- Might have to change the knife angle. Try cutting an "empty" paraffin
block (make a block with no tissue), and increase and decrease the knife
angle, until you can get sections without wrinkles from the plain block of
paraffin.
- If all the above fail, float the section in a container of 25% alcohol,
then pick the section up on a slide, drain the slide of the alcohol, and
slower lower the slide almost horizontal into the warm water flotation bath.
The folds need to be parallel to the surface of the water bath, not
perpendicular, in order for them to be pulled out by the difference in
surface tension between the water and the alcohol. If 25% alcohol is ripping
the section apart when laid on the water, try a lower percent of alcohol.
Let us know what worked for you, so we all can learn.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of cynthia
haynes
Sent: Wednesday, October 05, 2005 5:14 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] 20 micron sections
Hello, everyone I am in need of information. I am cutting brain(human)tissue
at 6 microns and 20 microns. The 6 microns sections are perfect. The 20
microns sections are an nightmare(and I mean Elm street nightmare). Can
anyone suggest the best way to cut these sections without getting wrinkles
and folds.
I am at my wits end.
Thanks in advance
Cynthia Haynes H.T.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Thu, 6 Oct 2005 10:26:11 +0100
From: Stephen.Eyres <@t> sanofi-aventis.com
Subject: [Histonet] GFAP protocol for fluorescence
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<OF7D2F843D.5C06CC59-ON80257092.00338ADF <@t> sanofi-aventis.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
Does anyone have a GFAP protocol for a fluorescence method that they could
share?
Many thanks
Steve
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Message: 14
Date: Thu, 6 Oct 2005 04:55:39 -0500
From: "Sauer, Barb" <BSauer <@t> stjosephswb.com>
Subject: [Histonet] staining under hoods
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B2AFB6D326A8804182A0E81C097D6F1030EAE9 <@t> Newman.stjosephswb.com>
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Does anyone use a Labconco (or another)hood to do staining of H&E's? We recently moved into a new facility and when we moved none of our stains worked right. We have not completely ruled out the water change but we now have purchased stain(instead of making our own) and the stain is good. It would really be helpful to get feedback on who has the entire staining set up under a hood in comparison to only the xylene under the hood. We also have had the problem of a Labconco hood that we purchased that is supposed to move the height to adjust to different size people using it. It's a great concept but Labconco did not supply the ventilation tubing to make it moveable, thus we are stuck with an expensive hood that doesn't move. Any ideas???? Thanks for your help.
B. Sauer
Histology Department
Synergy Health / St. Joesph's Hospital
West Bend, WI 53095
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Message: 15
Date: Thu, 6 Oct 2005 07:51:04 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] staining under hoods
To: "Sauer, Barb" <BSauer <@t> stjosephswb.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<83AACDB0810528418AA106F9AE9B7F7E01304DE2 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="iso-8859-1"
Can you change the vent tubing to flexible dryer vent tubing?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sauer,
Barb
Sent: Thursday, October 06, 2005 5:56 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] staining under hoods
Does anyone use a Labconco (or another)hood to do staining of H&E's? We recently moved into a new facility and when we moved none of our stains worked right. We have not completely ruled out the water change but we now have purchased stain(instead of making our own) and the stain is good. It would really be helpful to get feedback on who has the entire staining set up under a hood in comparison to only the xylene under the hood. We also have had the problem of a Labconco hood that we purchased that is supposed to move the height to adjust to different size people using it. It's a great concept but Labconco did not supply the ventilation tubing to make it moveable, thus we are stuck with an expensive hood that doesn't move. Any ideas???? Thanks for your help.
B. Sauer
Histology Department
Synergy Health / St. Joesph's Hospital
West Bend, WI 53095
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Message: 16
Date: Thu, 6 Oct 2005 08:13:47 -0400
From: "Houston, Ronnie" <Ronnie_Houston <@t> bshsi.com>
Subject: [Histonet] amyloid in FNA smears
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<530361BF03351B4CAE5270A05D3037B506B71A6E <@t> bsrexms01.bshsir.com>
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What is the best fixation for smears from a fat-pad of a patient suspected
with having amyloidosis, to get optimal preservation for demonstration of
amyloid?
Thanks
Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax
ronnie_houston <@t> bshsi.com
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Message: 17
Date: Thu, 06 Oct 2005 07:14:55 -0500
From: "danaspears <@t> frontiernet.net" <danaspears <@t> frontiernet.net>
Subject: [Histonet] High Complexity Testing - IHC
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051006071455.wmqs0oc8scko4gw4 <@t> webmail.frontiernet.net>
Content-Type: text/plain; charset="ISO-8859-1"
Hello everyone!
I have been looking in the archives and am still a bit confused. I have
a friend who has asked me to find out who may perform IHC in her lab...
Would you say the following is correct as to who can perform IHC?
BS or MT/HTL
AS in science HT or MLT
Prior to 24 April 1995 HS graduate AND Military training (50 wks) and
classified as a Medical Laboratory Specialist
OR Graduate of
an accredited lab/histology program.
Based on the above, I am interpreting that I currently have several
techs that cannot perform IHC. Two are HT(ASCP), one HS/OJT the other
GED/OJT. Neither with any college, military training, nor attended an
accredited program. These two cannot perform the testing. I have two
trainees, one has an associates, is an MLT, and is currently sitting
for his HT. (I think he is okay, or does he have to wait to pass his
HT?). The last is another trainee that has not completed her degree
yet, so she has a ways to go yet. She will have her BS in May, then
will sit for her HTL. Once she has her BS, she meets the requirements.
Does all of this sound correct?
Thanks for any and all help!
------------------------------
Message: 18
Date: Thu, 6 Oct 2005 13:53:48 +0100
From: gillian.2.brown <@t> gsk.com
Subject: [Histonet] Metachromasia of mast cells in epoxy resin
sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF42103E16.F8AA848B-ON80257092.004531EF-80257092.0046CB36 <@t> gsk.com>
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Hi Histonetters,
would any one have experience of counting granules in mouse mast cells in
tissues which have been formaldehyde fixed, decalcified in a formic acid
based solution, post osmicated and embedded in epoxy resin? I'm taking 1
micron sections and looking to assess the 'degranulation' status of each
mast cell in terms of how many are still dark blue and how many are pink
ie based on the metachromatic properties of toluidine blue. I suppose my
question is will the formic acid have altered the dye binding properties
so that I'll get erroneous results? I'm going to experiment with tissue
that does not normally have this step but any insights now would be most
welcome.
Many thanks
Gill Brown
GlaxoSmithKline Medicines Research Centre,
STEVENAGE,
------------------------------
Message: 19
Date: Thu, 6 Oct 2005 13:57:11 +0100
From: "Prior, Andrew" <Andrew.Prior <@t> Smith-Nephew.com>
Subject: [Histonet] Histology Journals
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D16CB9D969B4D3119F0D009027B70BBF0B5944D4 <@t> yofs04.wound.san>
Content-Type: text/plain; charset="iso-8859-1"
I was wondering if anyone could recommend a good histology journal, that
covers a lot of the methods and equipment.
I've had a look at some journals on the web, but would appreciate some
feedback from regular readers on how useful you find them.
Many thanks
Andrew
Andrew Prior
Histologist
Smith & Nephew Research Centre
York Science Park
Heslington
York, YO10 5DF
UK
Andrew.Prior <@t> smith-nephew.com
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Message: 20
Date: Thu, 6 Oct 2005 06:25:24 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] 20 micron sections
To: cynthia haynes <naje1972 <@t> yahoo.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051006132524.26581.qmail <@t> web61220.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Try increasing the cutting angle; cutting more slow and cooling the cutting surface inmediately before the section you are going to take.
cynthia haynes <naje1972 <@t> yahoo.com> wrote:Hello, everyone I am in need of information. I am
cutting brain(human)tissue at 6 microns and 20
microns. The 6 microns sections are perfect. The 20
microns sections are an nightmare(and I mean Elm
street nightmare). Can anyone suggest the best way to
cut these sections without getting wrinkles and folds.
I am at my wits end.
Thanks in advance
Cynthia Haynes H.T.
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Message: 21
Date: Thu, 6 Oct 2005 06:32:56 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Histology Journals
To: "Prior, Andrew" <Andrew.Prior <@t> Smith-Nephew.com>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20051006133256.73707.qmail <@t> web61219.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Although it could be considered as a "biassed" opinion I would suggest for you to look at the Journal of Histotechnology from the National Society of Histotechnology.
Rene J.
"Prior, Andrew" <Andrew.Prior <@t> Smith-Nephew.com> wrote:
I was wondering if anyone could recommend a good histology journal, that
covers a lot of the methods and equipment.
I've had a look at some journals on the web, but would appreciate some
feedback from regular readers on how useful you find them.
Many thanks
Andrew
Andrew Prior
Histologist
Smith & Nephew Research Centre
York Science Park
Heslington
York, YO10 5DF
UK
Andrew.Prior <@t> smith-nephew.com
Confidentiality.
This electronic transmission is strictly confidential to Smith & Nephew and
intended solely for the addressee. It may contain information which is
covered by legal, professional or other privilege. If you are not the
intended addressee, or someone authorised by the intended addressee to
receive transmissions on behalf of the addressee, you must not retain,
disclose in any form, copy or take any action in reliance on this
transmission. If you have received this transmission in error, please notify
the sender as soon as possible and destroy this message.
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Message: 22
Date: Thu, 6 Oct 2005 06:41:49 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Nevada Society of Histotechnology
To: connie grubaugh <conniegrubaugh <@t> hotmail.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051006134149.25590.qmail <@t> web61225.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Connie:
Could you use this oportunity to ask the members of the Nevada Society to contribute with Tricks of the Trade to be shared by all the fellow histotechs?
This could be a great help for all!
Rene J. Buesa
connie grubaugh <conniegrubaugh <@t> hotmail.com> wrote:
The Nevada Society of HIstotechnology will be having their next
seminar Oct. 28th and 29th at Sunrise Hospital Auditorium in Las Vegas
Nevada.
There will be a Friday evening get together with the vendors. Drinks
and snacks will be provided. This will start at 5:30 pm.
Saturday sign in at 7 am. Coffee and rolls will be provided by Fisher
Scientific.
Meeting starts at 8 am.
First meeting will be Pam Marcum -Is Your Tissue Processor Fighting
You? Fight Back. This will be 3 ceu"s.
There will be a luncheon provided by Sakura and Cardinal Health. A
business
meeting will also be held at this time.
Afternoon meetings.
1 pm- Sandra L. Cummings, Putting the bug in Histotechnology, A basic
understanding of Microorganisms.
To follow Marianne Mailhoit- Exposure control plan for Blood Borne
Pathogens.
There will be no charge for this meeting.
Hope to see everyone there.
Vegas is the best place to be on Halloween weekend.
Please email me with any questions, or call 702-396-4079 or
702-938-3619.
Connie Grubaugh
Nevada Society of Histotechnology
Striving for Excellence Despite the Odds.
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Message: 23
Date: Thu, 6 Oct 2005 09:55:39 EDT
From: Debbiejsiena <@t> aol.com
Subject: [Histonet] Job Openings In Dallas, Texas
To: histonet <@t> pathology.swmed.edu
Message-ID: <bf.614bb967.307686db <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Good Morning All
Tissue Techniques In Dallas, Texas has several HT openings along with an
opening for a Lab tech. If you are the best and expect the best of Histology
personnel than this is the lab for you. We have work that allows people to be
creative and solve problems along with doing some rearch projects.If you would
like to be part of a winning team, please contact us.
Debbie Siena, cell: 520-360-3013, Fred Siena, cell: 817-228-8023, Office:
972-241-6277, Fax 972-241-4747, e-mail: tissuetech <@t> juno.com
------------------------------
Message: 24
Date: Thu, 6 Oct 2005 08:56:14 -0500
From: "Kapoor, Sue" <Sue.Kapoor <@t> uhsi.org>
Subject: [Histonet] CAP question
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<61E9F2400F53D5119CFC00508B44E33B02E9E1B7 <@t> khmcexch.uhsi.org>
Content-Type: text/plain; charset=iso-8859-1
Hi everyone,
is anyone familiar with CAP #ANP.22925
"For IHC tests that provide independent predictive/prognostic information,
does the patient report include info on specimen processing, the antibody
clone, and the scoring method used? (e.g., hormone receptor in breast
carcinoma, HER-2/neu, EGFR).
"The lab should periodically compare its patient results with published
benchmarks, and also evaluate interobserver variabitlity among the
pathologists in the lab".
how are others handling this??
Many, many thanks in advance,
Sue Kapoor, HT (ASCP)
Histology Coordinator
Kenosha Medical Center
Kenosha, WI
262-653-5570
------------------------------
Message: 25
Date: Thu, 06 Oct 2005 10:22:25 -0400
From: Pamela Marcum <pmarcum <@t> vet.upenn.edu>
Subject: [Histonet] Region II Meeting November 4th and 5th, 2005
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <6.1.1.1.2.20051006101724.01950860 <@t> mail.vet.upenn.edu>
Content-Type: text/plain; charset="iso-8859-1"; format=flowed
Region II is having a meeting!!
We are including a copy of the program for your review. If anyone would
like a copy of the full program with registration, cost, and directions
please contact me. The meeting is at the Hilton Valley Forge Hotel in King
of Prussia, PA - outside Philadelphia, PA.
Best Regards,
Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr. CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348
Phone - 610-925-6278
Fax - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu
Friday - November 4, 2005 - Region II NSH Fall Meeting
Registration 7:00AM to 8:00AM
WS #1- 8:00 to 11:30AM Muscle
talk! Barone
WS #2- 8:00 to 11:30AM Preparing for the IHC
Qualification Examination (ASCP Registry) Macrea
WS #3- 8:00 to 11:30AM Unlocking The Secrets
of Mohs' Grossing and
Cryosectioning Praet
WS #4- 8:00 to 9:30AM Chemicon's Advanced Tissue
Arrayer (ATA 100), tissue Microarrays, IHC Select
Reagents, and IHC Manual Select Staining for High
Through-Put Screening
Studies Schaub
Morning Break In Exhibits - 9:30 to 10:00AM
WS # 5- 10:00 to 11:30AM Confocal, Multi-Photon and
Deconvolution Microscopy in Clinical Diagnosis Atkins
WS #6- 10:00 to 11:30AM Microwave Tissue Fixation
And
Processing
Frei
Afternoon Workshops and Seminars
WS #7- 1:00 to 2:30PM Laboratory Management:
Problems and
Solutions
Billings
WS#8- 1:00 to 2:30PM What Every Histotech Should
Know About
Water
Macrea
Afternoon Break In Exhibits - 2:30 to 3:00PM
WS#9- 1:00 to 4:30PM Quality Assessment of
Special
Stains
Micciche
WS#10- 1:00 to 4:30PM From Amygdala to Arabidopsis:
Why's and How's of Vibrating Blade Microscopy Freeland
WS#11- 3:00 to 4:30PM Mummies: Ancient to Modern Wade
WS#12- 3:00 to 4:30PM Reagent Alcohol -
Can't Drink It - So What Is
It? Marcum
Saturday - November 5, 2005 - Region II NSH Fall Meeting
Registration 7:00AM to 8:00AM
WS#13- 8:00 to 9:30AM Are All Tissues Created Equal? Hughes
Research vs
Clinical Louro
WS#14- 8:00 to 9:30AM High Profile Cases
Let the Stress
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