[Histonet] mounting media for EGFP in liver
cbass <@t> bidmc.harvard.edu
Wed Oct 12 16:19:07 CDT 2005
I have used a viral vector to transduce liver and pancreatic tissues
in the mouse with EGFP and RFP. I have cut portions of the liver and
pancreas into small blocks and fixed overnight with NBF. What is the
best way to visualize the signal? I want to cut 30 micron sections,
as I only have access to a sliding microtome. Could someone
recommend a good, and hopefully inexpensive, mounting media for
fluorescence? I have a fairly strong signal but I am worried about
losing it if I use the wrong product. I am not very experienced with
fluorescence so I really don't know what to use.
Someone recommended Aqua PolyMount from polysciences.
Any suggestions would be appreciated.
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