[Histonet] mounting media for EGFP in liver

Caroline Bass cbass <@t> bidmc.harvard.edu
Wed Oct 12 16:19:07 CDT 2005


I have used a viral vector to transduce liver and pancreatic tissues  
in the mouse with EGFP and RFP.  I have cut portions of the liver and  
pancreas into small blocks and fixed overnight with NBF.  What is the  
best way to visualize the signal?  I want to cut 30 micron sections,  
as I only have access to a sliding microtome.  Could someone  
recommend a good, and hopefully inexpensive, mounting media for  
fluorescence?  I have a fairly strong signal but I am worried about  
losing it if I use the wrong product.  I am not very experienced with  
fluorescence so I really don't know what to use.

Someone recommended Aqua PolyMount from polysciences.

Any suggestions would be appreciated.


More information about the Histonet mailing list