[Histonet] remove grinding debris

[Rittman, Barry R]

Myriam.
Are you using water as a lubricant on the paper? If so remember that
Epon is somewhat hygroscopic. Can grind using a neutral oil.
If grinding dry then repeat the grinding on a frosted glass plate. This
often will remove a lot of the debris. Can first try a commercial
frosted slide or prepare your own using alumina.
Are you sure that the debris is not being introduced during staining?
Hydroxyapatite and other minerals can cause precipitates and also may
bind to some dyes. 
Barry


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Myri37 <@t> aol.com
Sent: Wednesday, October 12, 2005 10:56 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] remove grinding debris

Hello everyone,

I grind on abrasive sandpaper, sections of titanium implants embedded in
epon. Titanium is coated with a hydroxyapatite layer and soft tissue.
After staining with RBS (rapid bone stain), i see on slides a lot of
debris,i think they are grinding debris and titanium or hydroxyapatite
dust...
Does anyone have a process to remove these debris ?
Do you recommend a wash with a specific detergent solution after
grinding, does 0.1 % Zephiran chloride solution efficient ? 
Thank you very much for any advice.

Myriam 
NI  France

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