[Histonet] Sticky Slides

Vernon Dailey vd38 <@t> georgetown.edu
Fri Oct 7 08:18:28 CDT 2005

Hi Histonet,

I am doing an in situ PCR/Hybridization. I am working with some cells 
that were suspended in histogel, fixed in formalin, processed and 
embedded. I am having some trouble keeping the tissue on the slides 
through all of the incubations and wash steps. I am using silanized 
slides and baking the slides at 60 degrees for 1 hour prior to use. Does 
anyone have any suggestions as to how I can get these samples to stick 
to the slides? Secondly, would a cytospin preparation be more likely to 
stay on the slide? Thanks in advance.


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