[Histonet] Fixation using Modified Bouins and NBF

[John A. Kiernan]

What is the composition of the "modified Bouin's"
fixative, and how long did the specimens remain in
it? Back in the old days Bouin's was considered a
good fixative for some immunofluorescent applications,
especially localizing protein and peptide hormones.
The picric acid in the solution subdues much of the
autofluorescence.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
"Ahmed, T (Tanni)" wrote:
> 
> 
> Dear Histonetters,
> 
> I would like to know if fixation of avian bursa tissue with Modified
> Bouins fixative over 10% Neutral Buffered Formalin has any advantagous
> effect on immuno precipitation?
> 
> Thanks in advance,
> 
> Tanni
> 
> 
> 
> 
> 
> Tanni  S Ahmed
> 
> Scientific Officer - Histopathology, R&D
> 
> 
> 
> Intervet UK Ltd.
> 
> Walton Manor,
> 
> Walton, Milton Keynes,
> 
> Buckinghamshire,
> 
> MK7 7AJ, UK.
> 
> 
> 
> Tel. +44(0)1908 685552/685543
> 
> Fax +44(0)1908 685614
> 
> E-mail: tanni.ahmed <@t> intervet.com
> 
> 
> 
> 
> 
> 
> 
> --------------------------------------
> This message, including attachments, is confidential and may be privileged.
> If you are not an intended recipient, please notify the sender then delete
> and destroy the original message and all copies. You should not copy, forward
> and/or disclose this message, in whole or in part, without permission of
> the sender.
> --------------------------------------
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet