[Histonet] RE: Tissue fixation help

Lori Richey lrichey <@t> u.washington.edu
Tue Oct 4 11:48:20 CDT 2005

It's a common protocol to embed tissue in paraffiin after its been 
reviewed as a frozen section.  The tissue is frozen for quick diagnosis 
while the patient is still in surgery.  It is then melted down and 
submitted for paraffin sections.

Charles Scouten wrote:

>Just very curious.  Why would anybody paraffin embed after the tissue
>had already been frozen? Either way gets it hard enough to cut, which is
>the purpose of both procedures.
>Charles W.  Scouten, Ph.D. 
>5918 Evergreen Blvd. 
>St. Louis, MO 63134 
>Ph: 314 522 0300 x 342
>FAX  314 522 0377 
>cwscouten <@t> myneurolab.com 
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
>Sent: Monday, October 03, 2005 2:46 PM
>To: 'C.M. van der Loos'; Histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] RE: Tissue fixation help
>I too have thawed tissue in rt formalin and then processed and embedded
>in paraffin which good morphology and IHC results (haven't tried with
>antibodies to phosphorylated sites but other IHC has been fine, it all
>depends on if the samples was properly frozen in the first place)  I
>tried to do this with some heart tissue which had just been placed in a
>-80dc freezer with out snap freezing in OCT and it was awful, but the
>frozen section was bad as well.
>Patsy Ruegg, HT(ASCP)QIHC
>IHCtech, LLC
>Fitzsimmons BioScience Park
>12635 Montview Blvd. Suite 216
>Aurora, CO 80010
>wk email pruegg <@t> ihctech.net
>web site www.ihctech.net
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>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of C.M. van
>der Loos
>Sent: Sunday, October 02, 2005 11:43 PM
>To: Histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] RE: Tissue fixation help
>   Angela,
>   Long  time  ago  we thawed a  frozen  tissue  sample directly in
>   temp.)  NBF  (24-48 hs). Morphology was kept surprisingly well.
>   at that time phosphorylated antibodies were not even existant....
>   Chris van der Loos, PhD
>   Dept. of Pathology
>   Academic Medical Center M2-230
>   Meibergdreef 9
>   NL-1105 AZ Amsterdam
>   The Netherlands
>   phone:  +31 20 5665631
>   Date: Fri, 30 Sep 2005 08:25:24 -0400
>   From: Angela McNabola <angela.mcnabola.b <@t> bayer.com>
>   Subject: [Histonet] Tissue fixation help
>   To: [1]Histonet <@t> lists.utsouthwestern.edu
>   Good Morning,
>   Does  anybody  on  the histonet have any experience with taking
>   that has
>   been snap frozen in minus 80, and then "thawed" out and formalin
>   and
>   paraffin embedded?
>   I  won't  get into the reasons why we have to do this, but does
>   have a
>   protocol  (or just slowly thaw it out and fix it??).  How did your
>   turn out,
>   especially  when  using  phosphorlyated  antibodies?  Did you get
>   morphology of
>   your tissue?
>   thanks in advance!
>   Angela
>   Angela McNabola, MS HT(ASCP)SLS, QIHC
>   Bayer Healthcare
>   400 Morgan Lane
>   West Haven, CT 06516
>   203-812-5001
>   angela.mcnabola.b <@t> bayer.com
>   1. mailto:Histonet <@t> lists.utsouthwestern.edu
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