[Histonet] RE: Tissue fixation help
Stephen Peters M.D.
petepath <@t> yahoo.com
Mon Oct 3 20:39:59 CDT 2005
Pretty much all of the frozen sections done in hospital pathology departments are then
thawed and paraffin embedded. Some call this the frozen section control. I have some a
few pics on my web site that show freeze artifact next to the frozen controls. About
midway down this page.
The nuclear chromatin is a bit less defined and overall have a slightly smudged
quality compared to tissue the not previously frozen. The actual ice crystal artifact seems
to dissipate after processing the tissue. As far as immunostains go, I have gotten
satisfactory results with previously frozen samples with maybe a bit more background.
The finicky ones are probably more finicky. How do others feel about immunos on frozen controls?
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