[Histonet] RE: Tissue fixation help

[Stephen Peters M.D.]

Pretty much all of the frozen sections done in hospital pathology departments are then
thawed and paraffin embedded. Some call this the frozen section control. I have some a 
few pics on my web site that show freeze artifact next to the frozen controls. About 
midway down this page. 
http://pathologyinnovations.com/new_page_3.htm
The nuclear chromatin is a bit less defined and overall have a slightly smudged 
quality compared to  tissue the not previously frozen. The actual ice crystal artifact seems 
to dissipate after processing the tissue. As far as immunostains go, I have gotten
 satisfactory results with previously frozen samples with maybe a bit more background. 
 The finicky ones are probably more finicky. How do others feel about immunos on frozen controls?
 
Stephen