Well said on RE: [Histonet] weak antibody signal

[Gayle Callis]

Patsy,

You couldn't have said it better!!!!   A rat kit i.e. for rat antimouse 
antibody IHC would be nice too and still on my hopechest/wish list.

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717

At 08:36 AM 11/23/2005, you wrote:
>What I do for goat primaries is to use a rab. Anti-goat secondary and then
>apply the rabbit labeled polymer (I use DAko Envision), it works a charm,
>with a very strong signal.  Invitrogen sent me a goat labeled polymer to try
>but it did not do well in my hands.  Dako has been promising me goat labeled
>polymer for years but so far I haven't seen it, most of these companies seem
>to still be more interested in clinical than research IHC.
>Patsy
>
>Patsy Ruegg, HT(ASCP)QIHC
>IHCtech
>12635 Montview Blvd. #216
>Aurora, CO 80010
>720-859-4060
>fax 720-859-4110
>pruegg <@t> ihctech.net
>www.ihctech.net
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
>Sent: Tuesday, November 22, 2005 11:45 AM
>To: Till, Renee
>Cc: histonet
>Subject: Re: [Histonet] weak antibody signal
>
>DAKO sells an LSAB+ kit (labeled streptavidin-biotin-HRP) that is against
>mouse, rabbit, AND goat IgGs.  I use it for my goat primary antibodies and
>it works well.
>
>Jan Shivers
>U of MN Vet Diag Lab
>shive003 <@t> umn.edu
>
>----- Original Message -----
>From: "Till, Renee" <TillRenee <@t> uams.edu>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Sent: Tuesday, November 22, 2005 10:44 AM
>Subject: [Histonet] weak antibody signal
>
>
>We have a BTEB1 goat polyclonal antibody that is staining very weakly
>using a Vectastain kit and DAB. I personally like to order my
>secondaries and streptavidin separate, that way I can get F(ab')2
>fragements or what have you, if I want. I was going to suggest trying
>this, as it is has worked better for me on some stains than the Vector
>kits. Is there anything else that could be done to boost the signal? I
>had thought of CSA kits and the like, but from what I can tell the Dako
>kit at least isn't for goat. Would it work better perhaps using
>fluorescence instead of DAB?
>
>
>
>Renee' Till, HT
>
>
>
>Arkansas Childrens Nutrition Center
>
>1120 Marshall
>
>Little Rock, AR
>
>72202
>
>
>
>(501) 364-2774
>
>
>
>
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