[Histonet] weak antibody signal

[patsy ruegg]

What I do for goat primaries is to use a rab. Anti-goat secondary and then
apply the rabbit labeled polymer (I use DAko Envision), it works a charm,
with a very strong signal.  Invitrogen sent me a goat labeled polymer to try
but it did not do well in my hands.  Dako has been promising me goat labeled
polymer for years but so far I haven't seen it, most of these companies seem
to still be more interested in clinical than research IHC.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: Tuesday, November 22, 2005 11:45 AM
To: Till, Renee
Cc: histonet
Subject: Re: [Histonet] weak antibody signal

DAKO sells an LSAB+ kit (labeled streptavidin-biotin-HRP) that is against
mouse, rabbit, AND goat IgGs.  I use it for my goat primary antibodies and
it works well.

Jan Shivers
U of MN Vet Diag Lab
shive003 <@t> umn.edu

----- Original Message ----- 
From: "Till, Renee" <TillRenee <@t> uams.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 22, 2005 10:44 AM
Subject: [Histonet] weak antibody signal


We have a BTEB1 goat polyclonal antibody that is staining very weakly
using a Vectastain kit and DAB. I personally like to order my
secondaries and streptavidin separate, that way I can get F(ab')2
fragements or what have you, if I want. I was going to suggest trying
this, as it is has worked better for me on some stains than the Vector
kits. Is there anything else that could be done to boost the signal? I
had thought of CSA kits and the like, but from what I can tell the Dako
kit at least isn't for goat. Would it work better perhaps using
fluorescence instead of DAB?



Renee' Till, HT



Arkansas Childrens Nutrition Center

1120 Marshall

Little Rock, AR

72202



(501) 364-2774




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