[Histonet] oil red o
Bryan Llewellyn
llewllew <@t> shaw.ca
Mon Nov 21 11:06:34 CST 2005
The ending "er" in English denotes a person (or thing) who/which does an
action. To "bug" is a colloquial expression meaning to irritate, so a
"bugger" (the letter g is doubled to preserve the short vowel sound) would
be someone (or something) who irritates someone else. Is there another
meaning?
Bryan
----- Original Message -----
From: "Rogerson Kemlo (ELHT) Pathology" <Kemlo.Rogerson <@t> elht.nhs.uk>
To: "Kristen Broomall" <kbroomal <@t> NEMOURS.ORG>; "Sally Prouty"
<prouty27 <@t> msn.com>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, November 21, 2005 8:28 AM
Subject: RE: [Histonet] oil red o
"Quite a bugger" I quote.
Do you know what one is? It doesn't seem to fit the context in which you
are using it. Are you using it as a noun or a verb?
I'm sure you aren't talking about Sally.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kristen
Broomall
Sent: Monday, November 21, 2005 4:19 PM
To: 'Sally Prouty'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] oil red o
Make sure that you are not pressing (at all!) on your coverslip, even to
get
out air bubbles. The ORO will come right out of the fat cells. Quite a
bugger.
Kristen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sally
Prouty
Sent: Sunday, November 20, 2005 11:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] oil red o
I am trying to locate a protocol for a consistant Oil red O stain. I
don't
know if it is in coverslipping where the stain is moving off the fat
cells
or in my differentiation step. I am staining on frozen skeletal muscle
sections. Any suggestions would be helpful. Thank you,
Sally
Sally J. Prouty
HHMI/University of Iowa
Kevin Campbell Laboratory
400 EMRB
Iowa City, Iowa 52246
319-335-6944
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