[Histonet] oil red o

[Rogerson Kemlo (ELHT) Pathology]

"Quite a bugger" I quote.

Do you know what one is? It doesn't seem to fit the context in which you
are using it. Are you using it as a noun or a verb?

I'm sure you aren't talking about Sally.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kristen
Broomall
Sent: Monday, November 21, 2005 4:19 PM
To: 'Sally Prouty'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] oil red o

Make sure that you are not pressing (at all!) on your coverslip, even to
get
out air bubbles. The ORO will come right out of the fat cells. Quite a
bugger.

Kristen

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sally
Prouty
Sent: Sunday, November 20, 2005 11:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] oil red o


I am trying to locate a protocol for a consistant Oil red O stain. I
don't 
know if it is in coverslipping where the stain is moving off the fat
cells 
or in my differentiation step. I am staining on frozen skeletal muscle 
sections. Any suggestions would be helpful. Thank you,

Sally


Sally J. Prouty
HHMI/University of Iowa
Kevin Campbell Laboratory
400 EMRB
Iowa City, Iowa 52246
319-335-6944

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