[Histonet] oil red o

[Anne Van Binsbergen]

I did ORO on musce bx's for years - successfully - try the method in
Bancroft and when you coverslip, be sure to use AQUEOUS mountant 
Annieinarabia

-----Original Message-----
From: Sally Prouty [mailto:prouty27 <@t> msn.com] 
Sent: Monday, November 21, 2005 8:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] oil red o

I am trying to locate a protocol for a consistant Oil red O stain. I
don't 
know if it is in coverslipping where the stain is moving off the fat
cells 
or in my differentiation step. I am staining on frozen skeletal muscle 
sections. Any suggestions would be helpful. Thank you,

Sally


Sally J. Prouty
HHMI/University of Iowa
Kevin Campbell Laboratory
400 EMRB
Iowa City, Iowa 52246
319-335-6944

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