[Histonet] Auto stainer IHC/SS

[Patsy Ruegg]

I use the reg. DakoAutostainer and have done some special stains on it, I
mostly use it for IHC and I have not ever had a cross contamination problem
even though it does not have disposable tips it uses one probe for
everything and cleans it with a sonicator between each reagent.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
pathrm35 <@t> adelphia.net
Sent: Friday, November 18, 2005 2:17 PM
To: LewisS <@t> pediatrics.ohio-state.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Auto stainer IHC/SS

 Sarah,
We purchased the Biogenex i6000 about 5 months ago and have had good results
with it. We run special stains (alcian blue/pas and PAS for fungus) along
with our immunos (mostly Melan A and S-100's). We are using about 17 of
their antibodies. The antigen retrieval system is easy to use and
consistant. The Biogenex rep., Alan Younger is very good and is a pleasure
to work with. 
We wanted to demo the Dako stainer but the Dako rep. was going to charge us
$1,700.00 shipping fee for the demo. My Dr. said "No Way".

Ron Martin, BS, HT (ASCP) HTL
---- "Lewis wrote: 
> Just a quick question in regards to the Autostainer Plus from Dako. I am
currently looking to purchase an Automated Immuno Stainer. I have demoed The
i6000, Mozaic, and the Nemesis.  I will soon demo the Autostainer Plus from
Dako.  They claim to be able to do special stains and IHC. Are any of you
running specials on your Autostainer plus?  Any thoughts on this?  Also,  If
you have any experience with any of the units any feedback would be
appreciated!!!  The i6000 also claims to do specials as well,  I only ran a
few,  I never tried to run IHC and specials together on the same run.(They
have disposable pipette tips which eliminates the cross contamination
issue.)      Any thoughts would be great!!! Thanks in advance!!!           
> 
> Sarah E. Lewis
> Histotechnician
> Childrens Research
> Center for Gene Therapy
> 700 Childrens Dr Rm WA3112
> Columbus OH 43205
> (614)-722-2204
> LewisS <@t> ccri.net
> 
>  
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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