[Histonet] formalin precipitate/Iron artifact

[Rogerson Kemlo (ELHT) Pathology]

Could it be from the needle? Are needles made of iron? Am I talking
rubbish?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Saundra
Johnson
Sent: 18 November 2005 12:41
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] formalin precipitate/Iron artifact

I am having a problem with needle core liver Bx.  The iron stains has a
funny blue artifact staining around the peripheral.  Bone marrows and
other
tissues with Iron stains do no exhibit this.  We recently started
recycling
our zink formalin with Creative Waste Managment's filitering system but
make
the correct adjustments as instructed. Additionally, there is a slight
precipate left in the retort chamber at the end of processing,even after
the
clean cycle. We have a 50% alcohol after the formalin and then go to
95%.
Any suggestions with these two problems and are they related to
recycling of
formalin

Saundra Johnson
Histology-IHC Supervisor
Sarasota Pathology



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