[Histonet] Slide Adhesion;toenail for fungus stain

[Patsy Ruegg]

It is really tuff to get toe nails and cartilage to stay on the slide,
especially if you use heat when staining.  My very best method includes
pre-coating slides with a 5% elmers glue solution, let the slides airdry
very well after coating, I usually airdry overnight.  Use these slides to
pick up your sample and then airdry them with the section on them again
overnight.  To melt the paraffin I place the airdryed sections on a flat
slide warming plate at 55dc for 10 min., followed again by complete
airdrying.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Christine
Tambasco
Sent: Thursday, November 17, 2005 11:15 AM
To: jr <@t> infosight.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide Adhesion;toenail for fungus stain


   Dear John,

   I  have  a  lot  of  trouble  getting  toenails  to stay on the slide,
   especially  doing  the GMS stain for fungus. I have used Poly-L-Lysine
   coated  slides,  probe  slides  and  even Elmers glue. I would be very
   interested in a slide that could keep the toenail attached. Thanks for
   R&R.


   Christine Tambasco, HT (ASCP)

   St. Mary's Hospital Laboratory

   Amsterdam, New York 12010

   ph-518-841-7287
       ______________________________________________________________

     From:  John Robertson <jr <@t> infosight.com>
     To:  histonet <@t> lists.utsouthwestern.edu
     Subject:  [Histonet] Slide Adhesion
     Date:  Wed, 16 Nov 2005 08:42:57 -0500
     >We are interested in seeing if a new slide coating method has
     >advantages  for  the  adhesion  of "difficult" sections. It should
     work
     >well  in  all  applications  and  ultimately  be  less costly than
     existing
     >  charged slides.
     >If  you  have  an  especially difficult adhesion problem , I would
     like
     >to  hear  from  you  and  offer  you  a  few prototype samples for
     testing.
     >Thanks
     >
     >John
     >
     >
     >
     >
     >John Robertson   P.E. Ph.D.         jr <@t> infosight.com
     >CEO  InfoSight Corporation          http://www.infosight.com     "
     We
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