[Histonet] Slide Adhesion;toenail for fungus stain

[Rene J Buesa]

If the toenails are well processed (which in its turn will require an alkaline "digestion" before processing until the toenail is pliable) they will usually stay in the positive slide.
  Have you tried adding carpenter's yellow glue mixed in the water bath (about 0.5% sol.)?
  Another thing, we used PAS to detect fungi (they stain fantastic) GMS is too "violent" for this type of so "capriceous" specimen. Would you give it a try to PAS for fungi in trying to circunvent this problem?
  Just a thought!
  Rene J. 

Christine Tambasco <immrstambo <@t> hotmail.com> wrote:
  
Dear John,

I have a lot of trouble getting toenails to stay on the slide,
especially doing the GMS stain for fungus. I have used Poly-L-Lysine
coated slides, probe slides and even Elmers glue. I would be very
interested in a slide that could keep the toenail attached. Thanks for
R&R.


Christine Tambasco, HT (ASCP)

St. Mary's Hospital Laboratory

Amsterdam, New York 12010

ph-518-841-7287
______________________________________________________________

From: John Robertson 
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slide Adhesion
Date: Wed, 16 Nov 2005 08:42:57 -0500
>We are interested in seeing if a new slide coating method has
>advantages for the adhesion of "difficult" sections. It should
work
>well in all applications and ultimately be less costly than
existing
> charged slides.
>If you have an especially difficult adhesion problem , I would
like
>to hear from you and offer you a few prototype samples for
testing.
>Thanks
>
>John
>
>
>
>
>John Robertson P.E. Ph.D. jr <@t> infosight.com
>CEO InfoSight Corporation http://www.infosight.com "
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