[Histonet] hard tissue distortion

[louise renton]

Helo all,

I've been asked this question by a postgrad and I wasn't able to
answer it to my satisfaction. The question was - how much distortion
occurs in tissue (in this case teeth) that have been fixed in 70%
alcohol, processed  by hand and embedded in MMA?, Sections were cut at
6um, stained free floating and mounted in Entellan on glass slides.
Any thoughts/references?  I would be most grateful for anything The
post grad is doing histometry on healing furcation defects, and is
wondering if there are any variables that he needs to mentionor take
into consideration.



Best regards


--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"....onwards through the fog!"