[Histonet] RE: aqueous coverslipping medium

[C.M. van der Loos]

   Paul,

   The media you call the "liquid coverslip" is in fact a kind of plastic
   polymer  that  can  be  mounted up organically after polymerisation. I
   experienced  that without this additional organic mounting the plastic
   polymer will fall off after some years. If this happens you can remove
   the  plastic  completely  by  soaking  the  slide  in luke warm water.
   Then "liquid-coverslip" again.

   Although  most  of  those  products  state in their data sheet that it
   should  be  used  without a  coverslip,  it also  works  fine  WITH  a
   coverslip. The only thing you should not do is harden at 60-70C as you
   normally  do  (it  causes air bubbles!). Just leave the slides at room
   temp.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Wed, 16 Nov 2005 12:40:06 -0500
   From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
   Subject: [Histonet] aqueous coverslipping medium
   To: "'histonet <@t> lists.utsouthwestern.edu'"
   What brand do you prefer for aqueous mounting medium to be used with a
   coverslip?  I have several good hydrocarbon-based coverslipping media,
   and
   also  some good aqueous media to be used without coverslips (so-called
   liquid
   coverslip),  but  I  don't  have  an aqueous coverslipping medium I am
   completely
   satisfied  with.   Applications would include immunofluorescence and a
   few
   enzyme  stains,  as well as lipid stains and other techniques that are
   not
   compatible  with  hydrocarbon-based  media.   Are there any such media
   that
   harden after application, to produce a permanent slide?