[Histonet] any suggestions?

[Pamela Marcum]

Thanks, Jennifer.   Pam

At 11:03 AM 11/10/2005, Hofecker, Jennifer L wrote:
>Well said Pam and Gayle!
>I know that every time I have a problem, my first thought is "ask 
>histonet" not because I am lazy or because I can't look it up.  I think 
>first of histonet because of the diversity of our listmembers and their 
>experience.  Just because a publication lists the steps of a protocol, 
>doesn't mean it will work.  I would rather get my info from the ones who 
>make these protocols work.
>On the other hand, every time I am about to post a query, I have to read 
>it 6 times to make sure there isn't anything in the post that I will be 
>chastised for.  While I agree that we should not "post with reckless 
>abandon", I do not think we should be terrified of backlash for asking how 
>to do something we've never done before, especially if it affects patient 
>care, whether directly or indirectly.
>
>Well, I am going to put on my bullet proof vest!
>Thanks for listening,
>Jennifer
>
>
>
>Jennifer Hofecker, HT (ASCP)
>Vanderbilt University Medical Center
>Division of Neuropathology
>(615) 343-0083
>(615) 343-7089 fax
>
>Thank You Gayle!!  It is sometimes easy to forget not everyone has access
>to the Internet or a way to even place a message on HistoNet.  We are
>attempting to assist people who are learning and while some may seem to be
>using HistoNet as way to avoid looking anything up the majority are asking
>questions that are not readily accessible or in an area  like murine animal
>models that is more difficult.  I have no problem with this and will answer
>most questions off line if it is a more esoteric  question.  I am surprised
>that the person with the issue about Rene's assistance for her friend is
>also the same Rene who has been asking all of us to contribute to Tricks of
>the Trade.
>
>Pamela A Marcum
>Special Procedures Manager
>Bone Histology
>UPENN Vet School
>
>
>At 05:33 PM 11/9/2005, Gayle Callis wrote:
> >Rene,
> >
> >Sorry to disagree, and I certainly did not understand what YOU meant by
> >"changing his ways"?  I couldn't see many changes here, other than some
> >refinement of his IHC method.  Many people working with murine CD marker
> >IHC often need a little help with murine  animal model IHC.   Since our
> >lab works with murine CD marker IHC approx. 95% of the time, on frozen
> >sections etc per the inquiry, we are happy to sleuth problems.  The
> >questions were valid ones for learning/refining of his techniques and
> >being a mousie person, I didn't have a problem with the inquiry.
> >
> >Some people (including a lot of experts in our field) do NOT want to be on
> >Histonet and second hand messaging certainly doesn't bother me as long as
> >more details are provided.  I frequently ask questions on Histonet to
> >access information for others in my department - that's part of my job.
> >Your colleague is welcome to email privately if you or they wish.
> >
> >Gayle Callis
> >Research Histopathology Supervisor
> >Veterinary Molecular Biology
> >Montana State University - Bozeman
> >PO Box 173610
> >Bozeman MT 59717-3610
> >406 994-6367
> >406 994-4303 (FAX)
> >
> >
> >
> >
> >
> >
> >- At 02:28 PM 11/9/2005, you wrote:
> >>With all due respect I think that your colleague's procedure is a royal 
> mess.
> >>On the other hand it seems that he is not very willing to change his
> >>ways, so the only thing I have to tell you (for him) is: good luck! He
> >>should be the one doing the asking, and not you!
> >>Rene J.
> >
> >Gayle Callis
> >Research Histopathology Supervisor
> >Veterinary Molecular Biology
> >Montana State University - Bozeman
> >PO Box 173610
> >Bozeman MT 59717-3610
> >406 994-6367
> >406 994-4303 (FAX)
> >
> >
> >
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>
>
>
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Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu