[Histonet] H & E staining on frozen sections
Tony Henwood
AnthonyH <@t> chw.edu.au
Thu May 26 18:50:01 CDT 2005
Alternatively, you could try Kiernan's Chromoxane cyanine R. This solution
stains in 3 minutes and gives a H&E picture. It is an acidic aqueous
solution containing Chromoxane cyanine R (CI 43820) and ferric chloride.
Reference in his book.
Another technique is to do a Diff quik on ethanol fixed frozen sections.
This will also give a H&E like picture.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: Lee & Peggy Wenk [mailto:lpwenk <@t> sbcglobal.net]
Sent: Thursday, 26 May 2005 8:53 PM
To: 'wen eng'; 'histonet'
Subject: RE: [Histonet] H & E staining on frozen sections
H&E is always a 2 step stain (Hematoxylin in one step, Eosin in another,
with lots of other solutions between).
Any chance you were doing a Giemsa? Or a Paragon - A basic fuchsin/toluidine
blue combination? These are both one step.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
Lee & Peggy Wenk
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of wen eng
Sent: Wednesday, May 25, 2005 3:00 PM
To: histonet
Subject: [Histonet] H & E staining on frozen sections
Dear histonetters,
Long time ago I used a H&E stain solution on frozen sections. I remember
it's a one step staining. But I couldn't find it now. Does anybody know
where I can order it?
Thanks in advance!\
Wen
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