[Histonet] NADH stain

[Tony Henwood]

Patsy,

The NADH probably refers to the enzymatic demonstration of Nicotinic Acid
Adenine Dinucleotide Dehydrogenase. It is used for the demonstration of
mithochondria especially in frozen sections of muscle biopsies.

The principle of the stain: The oxidation of NADH can be catalysed by flavin
enzymes (formerly called diaphorases). NADH-TR is a flavin enzyme which
takes up hydrogen reversibly and transfers it to the acceptor, a tetrazolium
salt. The tetrazolium is converted to purple-blue formazan pigment marking
the site of enzyme activity.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net] 
Sent: Thursday, 26 May 2005 4:13 AM
To: 'Histonet'
Subject: [Histonet] NADH stain


A pathologist is asking for a stain, possibly IHC he is calling NADH, does
this mean anything to anyone?
Patsy
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
viruses.
**********************************************************************