[Histonet] slide drying

[Edmondson David (RBV) NHS Christie Tr]

Has anyone suggested that the sections need melting on rather than just drying? or have you done this in the process described.       Shaking trapped water off before drying and then melting may be an issue if you have trapped amounts of water under the sections. Whether melted or just dried on.
David
Christie
Manchester UK

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Steven
Coakley
Sent: 24 May 2005 19:37
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] slide drying


I'm setting up a small Histo lab and using a 60C slide warmer to dry my slides.  I've been currently allowing them to air dry vertically for a couple of hours to overnight, then placing the slides on the slide warmer for an hour.  About 90% of the time the slides turn out ok, but some slide do show some detaching from the slide.  Both charges and non-charged slides with water bath adhesive seem to be involved.  I read a past artical on this site not recommending allowing slides to air dry as the section may entrap water?  Any suggestions.
 
Steve


		
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