[Histonet] help

Thu May 26 11:50:10 CDT 2005

I would like to be on the Histonet message list instead of the Histonet Digest. 

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To: histonet <@t> lists.utsouthwestern.edu
Sent: Tue, 24 May 2005 1:01:31 PM Eastern Daylight Time
Subject: Histonet Digest, Vol 18, Issue 34

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Today's Topics:

   1. Automated special stains (Bartlett, Jeanine)
   2. RE: Automated special stains (Dawson, Glen)
   3. RE: Automated special stains (Weems, Joyce)
   4. GOOD markers/pencils for cassettes (krat18 <@t> aol.com)
   5. Grossing (Heather.A.Harper <@t> pcola.med.navy.mil)
   6. Re: microwave processing (Kaye Ryan)
   7. freezing of specimens in surgery center (Elizabeth Chlipala)

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Message: 1
Date: Tue, 24 May 2005 11:58:15 -0400
From: "Bartlett, Jeanine" <jqb7 <@t> cdc.gov>
Subject: [Histonet] Automated special stains
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <CB857F6460D42E4AAEA195054A25406C0761625B <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain;   charset="us-ascii"

Hi everybody:

I'd like feedback on the automated special stain equipment being used
today.  I need something that does all the traditional stains but also
silvers...mainly GMS' and Steiner's.  I am most interested in an open
system so as not to be locked into using a particular vendor's reagents
if possible.

Vendors welcome to email as well.

Thanks,
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333

------------------------------

Message: 2
Date: Tue, 24 May 2005 11:01:39 -0500
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: RE: [Histonet] Automated special stains
To: "Bartlett, Jeanine" <jqb7 <@t> cdc.gov>,
    histonet <@t> lists.utsouthwestern.edu
Message-ID: <D2401DE71F59D71184BC00D0B7479B91E47A71 <@t> MILW_MAIL1>
Content-Type: text/plain;   charset="iso-8859-1"

Dako's Artisan Stainer is great and it has a 7 minute Steiner's that works
well.

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: Bartlett, Jeanine [mailto:jqb7 <@t> cdc.gov]
Sent: Tuesday, May 24, 2005 9:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Automated special stains

Hi everybody:

I'd like feedback on the automated special stain equipment being used
today.  I need something that does all the traditional stains but also
silvers...mainly GMS' and Steiner's.  I am most interested in an open
system so as not to be locked into using a particular vendor's reagents
if possible.

Vendors welcome to email as well.

Thanks,
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
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------------------------------

Message: 3
Date: Tue, 24 May 2005 12:04:47 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] Automated special stains
To: "Bartlett, Jeanine" <jqb7 <@t> cdc.gov>,
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <83AACDB0810528418AA106F9AE9B7F7EA45DC3 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain;  charset="iso-8859-1"

We have the DAKO Artisan and are pleased with the company service as well as the 
stains. 
GMS is good. We use the Warthin Starry - not sure they have a Steiner.

If there would be one change, I would wish that the stains could be started as 
ordered instead of by batch. 

Hope this helps!
Joyce

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Bartlett,
Jeanine
Sent: Tuesday, May 24, 2005 11:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Automated special stains

Hi everybody:

I'd like feedback on the automated special stain equipment being used
today.  I need something that does all the traditional stains but also
silvers...mainly GMS' and Steiner's.  I am most interested in an open
system so as not to be locked into using a particular vendor's reagents
if possible.

Vendors welcome to email as well.

Thanks,
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
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Thank you. Saint Josephs Health System, Inc.

------------------------------

Message: 4
Date: Tue, 24 May 2005 12:30:03 -0400
From: krat18 <@t> aol.com
Subject: [Histonet] GOOD markers/pencils for cassettes
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C72E94F8EEA07D-E44-6A28 <@t> mblk-d37.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

HELP!  HELP!  HELP!   We are having lots of problems with our markers (we're now 
using StatLab).  The writing smears and smudges when we fix tissues in PenFix, 
from a combination of the grease from the fat and the alcohol in the PenFix.   
What is everyone using to mark cassettes that will be fixed in alcohol-based 
fixatives?   ANY suggestions are welcome!   We cannot at this time buy a 
cassette marker machine.   Are there pencils that work well?   Our Tissue Tek 
pencils used to smear also, but maybe there are better ones?

------------------------------

Message: 5
Date: Tue, 24 May 2005 11:24:29 -0500
From: Heather.A.Harper <@t> pcola.med.navy.mil
Subject: [Histonet] Grossing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
    <807FE48C5A7CC940B973B58D32E701431991BE4E <@t> nhpens-exch1.pcola.med.navy.mil>

Content-Type: text/plain

   Currently I am grossing in the small specimens and I do all the
miscellaneous stuff, plus all my paper work that a supervisor has to do..
Grossing consumes 2 hrs of my morning and generally do not start until
between 8:30-9:00. My shift ends at 2:30. My co-worker works the night shift
(10-6) and embeds, cuts and stains (literally). I want to get some opinions.
Since I already accession, gross, etc... Is it fair for the pathologists to
want me also to do the recuts and special stains? I feel an enormous
pressure about this issue and I simply can't understand why some
pathologists act like it's so detrimental to be able to view slides and get
recuts/special stains the same day. I do not want to be rushed through
grossing to accommodate 2 of the 3 pathologists. Am I right or wrong for
feeling this way. I need feedback.

Heather A. Harper

Naval Hospital of Pensacola, FL

Supervisor Histology/Morgue

------------------------------

Message: 6
Date: Tue, 24 May 2005 12:47:52 -0400
From: "Kaye Ryan" <ryakay <@t> shands.ufl.edu>
Subject: Re: [Histonet] microwave processing
To: <histonet <@t> lists.utsouthwestern.edu>,    <JEllin <@t> yumaregional.org>
Message-ID: <s293229f.086 <@t> GW-FS1.SHANDS.UFL.EDU>
Content-Type: text/plain;   charset=US-ASCII

Hi Jesus,

We have been doing the microwave processing for a while now and have
seen no change in the IHC staining patterns at all.  As well, our
routine H&E was not altered in any way.  The TAT cannot be beat and the
cutting of the tissues is much easier.  I have only had this "chatter"
issue come up since a hospital  merged with our lab several months ago.

Kaye Ryan
Histology Manager/Educational Coordinator
Rocky Point Laboratories
265-0111, 72093

>>> "Jesus Ellin" <JEllin <@t> yumaregional.org> 05/24/05 11:35 AM >>>
I was wondering our Pathologist is wanting to go to this microwave
technology and we have budgeted for 2 new processors one microwave and
one that it the conventional type.  My question would be that how many
people out there are doing microwave processing and has anyone seen a
discrepancy with immunos or specials due to the microwave processing?

Jesus Ellin
Yuma Regional Medical Center

>>> "Kaye Ryan" <ryakay <@t> shands.ufl.edu> 05/24/05 04:53AM >>>
We have begun processing tissues using the microwave processor.  For
the
most part, everything we process turns out beautifully but we seem to
be
having a problem with small endoscopic biopsies.  We have begun seeing
chatter but it appears to only be in the neoplastic part of the biopsy.

I know that normally chatter is due to cutting techniques or
over-drying
of the tissue but we take the extra steps to ensure the blocks are
appropriately cut.  Since it seems to be localized to the neoplastic
area I was wondering if this could be due to the microwave processing.

We occasionally had this problem with routine processing but only
occasionally.  Everything else cuts very well.  Has anyone heard of or
experienced similar problems with microwave processing or can you
offer
some advice or tips as to how to troubleshoot this problem

Kaye Ryan
Histology Manager/Educational Coordinator
Rocky Point Laboratories
265-0111, 72093

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------------------------------

Message: 7
Date: Tue, 24 May 2005 10:58:53 -0600
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: [Histonet] freezing of specimens in surgery center
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000d01c56081$e0dd14c0$a7d48a80 <@t> AMY>
Content-Type: text/plain;   charset="us-ascii"

Hello Histonetters

I have a unique question.  We are currently starting to set up procedure
for collecting samples from a clinical trial.  The clinical trial
involves taking multiple synovial biopsies at a surgery center.  Since
portions of the samples need to be processed for frozen sections we
wanted to be able to freeze the specimens at the surgery center via
isopentane cooled liquid nitrogen.  We really do not want to have to
transport the multiple specimens back to the main lab prior to freezing
due to the time involved it would probably be 1-2 hours post biopsy
before we could freeze the samples.  The surgery center is questioning
the flammability of the isopentane.  Has anyone encountered anything
like this?  Any suggestions would be helpful.  Thanks in advance.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com

Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

------------------------------

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