[Histonet] Fish Eggs-Help

[Andrea Grantham]

Once I did some frog eggs which are probably similar in that the centers 
mostly fell out - I did frozens and also paraffin sections. Fortunately the 
investigator only wanted to observe the walls and not the center yolky 
part. I found that soaking them and sometimes using a little ammonia water 
or soapy water helped - I could then get a section or two before I had to 
resoak.
I don't have a processor that uses vacuum and pressure but I was wondering 
if that would have any better effects on processing?
Andi Grantham



At 02:25 PM 5/25/2005 -0400, Greg Dobbin wrote:
>Hello Everyone,
>Who among you have had happy experiences with
>fixation/processing/sectioning of unfertilized fish eggs??
>
>A graduate student here at the Vet College is trying to section
>paraffin blocks with Arctic char eggs. The eggs are falling apart on
>the water bath (or on the way to the waterbath). He only wants to be
>able to do H&E on (stepped) serial sections. Any advise? Any at
>all?
>
>For insatnce, would frozens be a better option? Is the fixtive choice
>important, i.e. are fish eggs like eyes- should he be using Bouins?
>
>I welcome any and all input. Thanks.
>Greg
>
>
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>Greg Dobbin
>Research Technologist,
>Pathology Lab,
>Atlantic Veterinary College, U.P.E.I.
>550 University Ave.
>Charlottetown, P.E.I.
>Canada,  C1A 4P3
>Phone: (902)566-0744
>Fax: (902)566-0851
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>Happiness is a journey, not a destination.
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html