[Histonet] Fish Eggs-Help

[Greg Dobbin]

Hello Everyone,
Who among you have had happy experiences with 
fixation/processing/sectioning of unfertilized fish eggs??

A graduate student here at the Vet College is trying to section 
paraffin blocks with Arctic char eggs. The eggs are falling apart on 
the water bath (or on the way to the waterbath). He only wants to be 
able to do H&E on (stepped) serial sections. Any advise? Any at 
all?

For insatnce, would frozens be a better option? Is the fixtive choice 
important, i.e. are fish eggs like eyes- should he be using Bouins?

I welcome any and all input. Thanks. 
Greg 


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Greg Dobbin 
Research Technologist,
Pathology Lab,
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
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Happiness is a journey, not a destination.