[Histonet] Removing OCT???

[Andrea T. Hooper]

Hi Katri,

I would expect substantial deleterious damage to the cytoarchitecture 
using this method, as freeze-thaw is generally to be avoided ... are 
your experiences different? I suggest that Cathy cut frozen sections 
and post-fix in 10% NBF for 10 min at RT as an alternative.

Thanks,
Andrea


At 10:06 PM -0400 5/23/05, Katri Tuomala wrote:
>Hi Cathy,
>Just put the OCT embedded tissue in 10% NBF and let come to room 
>temperature, change formalin and let fix appropriate time.
>
>Katri
>
>Katri Tuomala
>Hamilton, Ontario, Canada
>----- Original Message ----- From: "Catherine Stanecki" 
><cestanecki <@t> ucdavis.edu>
>>
>>I am new to staining tissues and I recently froze a bunch of tissues in
>>OCT.  However, I recently found out that it would be better to have some
>>sample fixed in formalin as well so you can use mutliple staining methods.
>>Is there a way to remove OCT from tissue samples without damaging the
>>tissue, or is the tissue forever stuck in tissue tek once you freeze it back?
>>
>>Thanks!
>>Cathy
>>

--