[Histonet] Removing OCT???

[Katri Tuomala]

Hi Cathy,
Just put the OCT embedded tissue in 10% NBF and let come to room 
temperature, change formalin and let fix appropriate time.

Katri

Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "Catherine Stanecki" <cestanecki <@t> ucdavis.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, May 23, 2005 8:19 PM
Subject: [Histonet] Removing OCT???


>
> I am new to staining tissues and I recently froze a bunch of tissues in
> OCT.  However, I recently found out that it would be better to have some
> sample fixed in formalin as well so you can use mutliple staining methods.
> Is there a way to remove OCT from tissue samples without damaging the
> tissue, or is the tissue forever stuck in tissue tek once you freeze it 
> back?
>
> Thanks!
> Cathy
>
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