[Histonet] re GFAP localisation in pwax sections
Carl Hobbs
carl.hobbs <@t> kcl.ac.uk
Tue May 24 02:02:57 CDT 2005
I also use Dako's Z334 anti GFAP. For me, a 1:2000 dilution followed by a
std Dako strepABCpx-DAB after TRIS pH10 HIER is optimum. Very good on pwax
sections of mouse, rat and chick and .....human! However, as was rightly
advised, conditions need to be optimised locally.
If you wish, have a look at some pics on this site, p4/5 in Immunohist
gallery
http://www.immunoportal.com
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