[Histonet] RE: mouse mammary glands

Margaryan, Naira NMargaryan <@t> childrensmemorial.org
Mon May 23 18:52:19 CDT 2005


Thanks a lot for all of your suggestions :-)

I appreciate your and our :-) business,

Naira

 

-----Original Message-----
From: Favara, Cynthia (NIH/NIAID) [mailto:cfavara <@t> niaid.nih.gov] 
Sent: Monday, May 23, 2005 6:09 PM
To: Margaryan, Naira; histonet
Subject: RE: [Histonet] (no subject)

 

Maria,

 

Some pertinent questions would be:

      How are you collecting the sample?

      What is the processing schedule?

 

      I have done some studies here with mouse mammary glands and have

collected the gland including the nipple. I have not had any problems
with

cutting.

 

We shaved the belly being careful around the nipple so as not to injure
the

tissue. Cut a thin section on either side of the nipple. Process using
ETOH

70%-2 hours, 80% -1 hour, 2 95% ETOH - 20 minutes each, 2 100% ETOH 20

minutes each, 3-Propar 30 minutes each, 4 paraffins 30 minutes each.
Embedd

as you would skin. In my hands most mouse tissue requires some soaking
prior

to cutting. Be sure the temp of your water bath is not to hot or the fat

beneath the skin will just explode. I find the temperature range to be

fairly narrow.

 

Hope this is helpful.

 

c

 

 

      

 

Cynthia Favara

NIAID/NIH/RML/LPVD

903 South 4th Street

Hamilton, MT 59840

406-363-9317

 

Disclaimer: 

The information in this e-mail and any of its attachments is
confidential

and may contain sensitive information. It should not be used by anyone
who

is not the original intended recipient. If you have received this e-mail
in

error please inform the sender and delete it from your mailbox or any
other

storage devices. National Institute of Allergy and Infectious Diseases
shall

not accept liability for any statements made that are sender's own and
not

expressly made on behalf of the NIAID by one of its representatives

 

 

-----Original Message-----

From: Margaryan, Naira [mailto:NMargaryan <@t> childrensmemorial.org] 

Sent: Monday, May 23, 2005 11:35 AM

To: histonet

Subject: [Histonet] (no subject)

 

Hi Histos!

 

 

 

I am having problems with cutting FFPE mouse nipple. Paraffin is cutting

well but small tissue just crushing. We can't collect samples.

 

Please help, any suggestion is appreciated.

 

 

 

Thanks,

 

Naira

 

 

 

Naira V. Margaryan, Ph.D, D.V.M.

 

Research Associate II

 

Children's Memorial Research Center

 

2300 Children's Plaza, Box 222

 

Chicago, IL 60614-3394

 

Tel: 773-880-4000/5-6740

 

Fax: 773-755-6594

 

nmargaryan <@t> childrensmemorial.org

<mailto:nmargaryan <@t> childrensmemorial.org> 

 

 

 

For Express Mail: 

 

CMRC, Room C.473

 

2430 N. Halsted Street

 

Chicago, IL  60614-4314

 

 

 

 

 

The contents of this e-mail message and any attachments are private and

confidential communications intended solely for the addressee(s) named
in

this message. If you are not the intended recipient of this message,
please

1) immediately notify the sender by reply e-mail and then delete this

message and its attachments and 2) do not read, use, distribute disclose
or

copy this message and/or any attachments. 

_______________________________________________

Histonet mailing list

Histonet <@t> lists.utsouthwestern.edu

http://lists.utsouthwestern.edu/mailman/listinfo/histonet



The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. 



More information about the Histonet mailing list