[Histonet] RE: mouse mammary glands

[Margaryan, Naira]

Thanks a lot for all of your suggestions :-)

I appreciate your and our :-) business,

Naira

 

-----Original Message-----
From: Favara, Cynthia (NIH/NIAID) [mailto:cfavara <@t> niaid.nih.gov] 
Sent: Monday, May 23, 2005 6:09 PM
To: Margaryan, Naira; histonet
Subject: RE: [Histonet] (no subject)

 

Maria,

 

Some pertinent questions would be:

      How are you collecting the sample?

      What is the processing schedule?

 

      I have done some studies here with mouse mammary glands and have

collected the gland including the nipple. I have not had any problems
with

cutting.

 

We shaved the belly being careful around the nipple so as not to injure
the

tissue. Cut a thin section on either side of the nipple. Process using
ETOH

70%-2 hours, 80% -1 hour, 2 95% ETOH - 20 minutes each, 2 100% ETOH 20

minutes each, 3-Propar 30 minutes each, 4 paraffins 30 minutes each.
Embedd

as you would skin. In my hands most mouse tissue requires some soaking
prior

to cutting. Be sure the temp of your water bath is not to hot or the fat

beneath the skin will just explode. I find the temperature range to be

fairly narrow.

 

Hope this is helpful.

 

c

 

 

      

 

Cynthia Favara

NIAID/NIH/RML/LPVD

903 South 4th Street

Hamilton, MT 59840

406-363-9317

 

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-----Original Message-----

From: Margaryan, Naira [mailto:NMargaryan <@t> childrensmemorial.org] 

Sent: Monday, May 23, 2005 11:35 AM

To: histonet

Subject: [Histonet] (no subject)

 

Hi Histos!

 

 

 

I am having problems with cutting FFPE mouse nipple. Paraffin is cutting

well but small tissue just crushing. We can't collect samples.

 

Please help, any suggestion is appreciated.

 

 

 

Thanks,

 

Naira

 

 

 

Naira V. Margaryan, Ph.D, D.V.M.

 

Research Associate II

 

Children's Memorial Research Center

 

2300 Children's Plaza, Box 222

 

Chicago, IL 60614-3394

 

Tel: 773-880-4000/5-6740

 

Fax: 773-755-6594

 

nmargaryan <@t> childrensmemorial.org

<mailto:nmargaryan <@t> childrensmemorial.org> 

 

 

 

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