[Histonet] (no subject)

Favara, Cynthia (NIH/NIAID) cfavara <@t> niaid.nih.gov
Mon May 23 18:08:50 CDT 2005


Maria,

Some pertinent questions would be:
	How are you collecting the sample?
	What is the processing schedule?

	I have done some studies here with mouse mammary glands and have
collected the gland including the nipple. I have not had any problems with
cutting.

We shaved the belly being careful around the nipple so as not to injure the
tissue. Cut a thin section on either side of the nipple. Process using ETOH
70%-2 hours, 80% -1 hour, 2 95% ETOH - 20 minutes each, 2 100% ETOH 20
minutes each, 3-Propar 30 minutes each, 4 paraffins 30 minutes each. Embedd
as you would skin. In my hands most mouse tissue requires some soaking prior
to cutting. Be sure the temp of your water bath is not to hot or the fat
beneath the skin will just explode. I find the temperature range to be
fairly narrow.

Hope this is helpful.

c


	

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

Disclaimer: 
The information in this e-mail and any of its attachments is confidential
and may contain sensitive information. It should not be used by anyone who
is not the original intended recipient. If you have received this e-mail in
error please inform the sender and delete it from your mailbox or any other
storage devices. National Institute of Allergy and Infectious Diseases shall
not accept liability for any statements made that are sender's own and not
expressly made on behalf of the NIAID by one of its representatives


-----Original Message-----
From: Margaryan, Naira [mailto:NMargaryan <@t> childrensmemorial.org] 
Sent: Monday, May 23, 2005 11:35 AM
To: histonet
Subject: [Histonet] (no subject)

Hi Histos!

 

I am having problems with cutting FFPE mouse nipple. Paraffin is cutting
well but small tissue just crushing. We can't collect samples.

Please help, any suggestion is appreciated.

 

Thanks,

Naira

 

Naira V. Margaryan, Ph.D, D.V.M.

Research Associate II

Children's Memorial Research Center

2300 Children's Plaza, Box 222

Chicago, IL 60614-3394

Tel: 773-880-4000/5-6740

Fax: 773-755-6594

nmargaryan <@t> childrensmemorial.org
<mailto:nmargaryan <@t> childrensmemorial.org> 

 

For Express Mail: 

CMRC, Room C.473

2430 N. Halsted Street

Chicago, IL  60614-4314

 



The contents of this e-mail message and any attachments are private and
confidential communications intended solely for the addressee(s) named in
this message. If you are not the intended recipient of this message, please
1) immediately notify the sender by reply e-mail and then delete this
message and its attachments and 2) do not read, use, distribute disclose or
copy this message and/or any attachments. 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




More information about the Histonet mailing list