[Histonet] GFAP - Rat

[Andi Kappeler]

Hi Joost
We did compare 6 different pretreatmeant variants and had best results with 
HIER (citrate) in a pressure cooker, although we did see some faint staining 
without HIER. It may also depend on the way (time, fixative) your tissue 
samples have been fixed. If you have the possibility, run a comparison 
yourself to be sure you have the protocol that works best with your 
material.
Best regards
Andi

----- Original Message ----- 
From: "Bruijntjes, J.P." <bruyntjes <@t> voeding.tno.nl>
To: "Andi Kappeler" <kappeler <@t> patho.unibe.ch>
Sent: Monday, May 23, 2005 5:35 PM
Subject: RE: [Histonet] GFAP - Rat


> Hi Andy
>
> Thanks for your reply. You use HIER pretreatment (citrate). Is it really
> necessary? Someone else recommended the same antibody and she did not
> use a HIER technique.
>
> Joost Bruijntjes
>
>
> -----Original Message-----
> From: Andi Kappeler [mailto:kappeler <@t> patho.unibe.ch]
> Sent: maandag 23 mei 2005 16:26
> To: Bruijntjes, J.P.; Histonet
> Subject: Re: [Histonet] GFAP - Rat
>
> Hi Joost
>
> We have been using DakoCytomation's polyclonal rb-a-GFAP, Z 0334, with
> quite
> good success on rat brains. Dilution was 1:500-1:1000, pretreatment:
> HIER
> (citrate pH 6.0, pressure cooker). Hope this helps.
>
> Best regards
> Andi Kappeler, Ph.D.
> Institute of Pathology, University of Bern, Switzerland
>
> ----- Original Message ----- 
> From: "Bruijntjes, J.P." <bruyntjes <@t> voeding.tno.nl>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Monday, May 23, 2005 3:37 PM
> Subject: [Histonet] GFAP - Rat
>
>
>> Hi all
>>
>>
>>
>> Is anyone of you working with rat brain and GFAP?
>>
>> I have noticed that many GFAP antibodies do cross react with rat
> tissue,
>> but maybe someone of you is working with rat tissue and does have a
>> preference to a particular antibody. If so, can you share this
> knowledge
>> with me?
>>
>>
>>
>> Joost Bruijntjes
>>
>> TNO Quality of Life
>>
>> Zeist Holland
>>
>>
>>
>>
>>
>>
>>
>>
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>
>
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