Sodium citrate. Was Re: [Histonet] (no subject)

[John Kiernan]

"No subject"  Grrrrr!

Trisodium citrate alone would make an unbuffered 
alkaline solution.
 
Some antigens need an alkaline retrieval solution,
and there are a few that require an acidic solution.
pH 6 is satisfactory for most, and is less likely
to remove the sections from the slides than a
solution of more extreme (especially alkaline)
pH.

For a short article about how antigen retrieval might 
work, look at the spring newsletter of Region IX of 
the NSH:
http://www.nshregionix.org/springn05ewsletter.pdf

John Kiernan
Anatomy, UWO,
London, Canada
_____________________________
eswary wrote:
> 
> hi,
> 
> does anyone know the advantage of using 0.01M sodium citrate over 0.01M
> citric acid buffer, pH 6, for antigen retrieval on paraffin embedded
> sections? also, is the sodium constituent important? would it matter if
> i used trisodium citrate instead of monosodium?
> 
> thanks.
> 
> et
> 
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