[Histonet] Luna's toluidine blue; also Ventana Benchmark XT m orphology

[Moklebust, Amanda C]

Regarding the morphology changes in your patient tissue.  You may want to
examine how the slides are dried.  I have worked in labs where controls were
precut and dried in a 37 degree oven overnight.  When we received an IHC
order,  we placed the patient tissue on the lower portion of a control
slide.   Then the slides were dried again.  I am not currently using a
Benchmark for IHC staining, but I thought those instruments were going to
include protocols for drying and deparaffinizing slides.  If your control is
already dried and your patient tissue still has water around it when it is
loaded on the instrument, it could be one avenue for more troubleshooting.
Good Luck, 

Amanda Moklebust
Seattle Cancer Care Alliance

 -----Original Message-----
From: 	Malam Jacqueline [mailto:Jacqueline.Malam <@t> rli.mbht.nhs.uk] 
Sent:	Wednesday, May 11, 2005 8:57 AM
To:	'histonet <@t> lists.utsouthwestern.edu'
Subject:	[Histonet] Luna's toluidine blue; also Ventana Benchmark XT
morphology

I would appreciate it if I could also be sent details of Luna's toluidine
blue stain for mast cells, and any others that are considered superior so I
can compare with our current method. Thanks!

Also - do any of you users of Ventana's Benchmark or Benchmark XT
autoimmunostainer have a problem with poor morphology after heat retrieval -
usually affecting collagen / fibro-fatty tissues, and also the occasional
section detachment? We trialled the Benchmark which was fine but the
Benchmark XT which we purchased a year ago produces this problem. It does
not affect our positive control tissue (or very little) and I have been
through every possibility within the department and with Ventana but to no
avail. All ideas / clues welcome!
Jacqui Malam
Royal Lancaster Infirmary



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