Heat inactivating serum Re: [Histonet] New rabbit antibody.

Wed May 11 12:02:24 CDT 2005

To heat inactivate complement found in pooled serums. Complement has been 
known to cause nonspecific background staining.  You can read more about 
this in free Immunochemical Staining Methods Manual, 3rd Edition, 
DAKO.  They have this on their website or you can access a hard copy.

At 09:48 AM 5/11/2005, you wrote:
>i am curious as this has come up with a flow cytometry protocol as well.
>why do you heat inactivate serum for a staining procedure?
>thanks a lot,
>dana

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)