[Histonet] RE: RVU units

Patti Loykasek ploykasek <@t> phenopath.com
Wed May 11 11:41:35 CDT 2005


I would like to see the answer posted on the histonet, too.

Patti Loykasek
PhenoPath Laboratories
Seattle, WA


> I need to set up two tests and need to assign RVU units to two
> CPT codes in order to get the tests I need in our billing system.  I
> understand this may be a touchy subject but if anyone is willing to
> share how many RVU units they assigned to the CPT codes for 88189 Flow
> cytometry interpretation and 88358 DNA ploidy Digital image analysis I
> would appreciate it greatly.
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> histonet-request <@t> lists.utsouthwestern.edu
> Sent: Wednesday, May 11, 2005 11:10 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 18, Issue 14
> 
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> 
> Today's Topics:
> 
>  1. Mast cell stain (Osborn, Sharon)
>  2. Slide labelers (Osborn, Sharon)
>  3. Re: Asbestos studies (Gayle Callis)
>  4. Control for Mast cells (Gayle Callis)
>  5. Picrosirius red stain (Jeffrey Wu)
>  6. RE: Control for Mast cells (Jim Staruk)
>  7. Re: mast cell in guinea pig (John Kiernan)
>  8. RE: Asbestos studies (Shirley Powell)
>  9. Final notice: Missouri Society for Histotechnology    Spring
>     Symposium (Johnson, Teri)
> 10. Master's of Histology (Farley, Sunni R)
> 11. job (cynthia haynes)
> 12. Re: Slide Labeller (MajorFocus <@t> aol.com)
> 13. Re: Mast cell stain (Laurie Reilly)
> 14. Re: job (Patti Loykasek)
> 15. Mast Cell/Giemsa clarification (HSRL)
> 16. RE: Master's of Histology (Kemlo Rogerson)
> 17. (no subject) (eswary)
> 18. EDTA (Bernadette Weston)
> 19. macrophages in mouse FFPE
>     (wasielewski.reinhard.von <@t> mh-hannover.de)
> 20. Gayle - floating petri dish snap freezing question
>     (Kristen Broomall)
> 21. RE: macrophages in mouse FFPE (Flynn, Evelyn)
> 22. New rabbit antibody... but what do I use as negative
>     (isotype) control? (- -)
> 23. RE: job (Margaryan, Naira)
> 24. Unsubscribe (Flores, Teresa)
> 25. Unsubscribe (Flores, Teresa)
> 26. Help!!! (vsailes <@t> nd.edu)
> 27. job listing (LINDA MARGRAF)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Tue, 10 May 2005 13:14:25 -0400
> From: "Osborn, Sharon" <sharon.osborn <@t> dnax.org>
> Subject: [Histonet] Mast cell stain
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <29B25753F6B1D51196110002A589D44402397F8E <@t> PALMSG30.us.schp.com>
> Content-Type: text/plain
> 
> Tom,
> 
> Luna's Toluidine Blue Method for Mast Cells is a superior demonstration
> of
> mast cells using a pink background.  These literally pop up under low
> power.
> The method is located on pages 311-312 of Luna's Histopathologic Methods
> and
> color Atlas of special Stains and Tissue Artifacts; 1992.  If you wish a
> copy of the procedure, private email me and I will send to you.
> 
> sharon osborn
> DNAX ScheringPlough BioPharma
> Palo Alto, CA
> 
> Subject: [Histonet] Giemsa Stain for Mast Cells
> Netters,
> I am looking for a Giemsa stain for Mast Cells that does not have a
> pinkish
> hue like the Gaffney Giemsa.  We are looking to have the Mast Cells pop
> out
> at us on low power.  Any suggestions/protocols?
> Thanks,
> Tom
> Tom Galati
> Laboratory Director
> HSRL, Inc.- A GLP Compliant Contract Laboratory
> 137 South Main Street
> Woodstock, Virginia  22664
> (540)459-8211
> Fax: (540)459-8217
> tomgalati <@t> hsrl.org
> www.hsrl.org <www.hsrl.org>
> 
> 
> 
> *********************************************************************
> This message and any attachments are solely for the intended recipient.
> If you are not the intended recipient, disclosure, copying, use or
> distribution of the information included in this message is prohibited
> -- Please immediately and permanently delete.
> 
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Tue, 10 May 2005 13:21:48 -0400
> From: "Osborn, Sharon" <sharon.osborn <@t> dnax.org>
> Subject: [Histonet] Slide labelers
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <29B25753F6B1D51196110002A589D44402397F8F <@t> PALMSG30.us.schp.com>
> Content-Type: text/plain
> 
> Cynthia,
> Contact your Leica representative and ask for a demonstration of
> their slide printers.  I think you will be pleasantly surprised as to
> the
> amount of information and quietness.  I have used several different
> slide
> labelers including the etch-a-sketch types and ink printer types; this
> one
> is the best of all I have used.  Its footprint is a little larger than
> the
> others and well worth it.  Sakura and Leica partnered on developing
> these;
> their main difference is in the software that is provided. Sakura uses
> off
> the shelf software while Leica specifically built the software on theirs
> and
> it is customizable for stand alone or for integration with MIS of an
> institution.  And, the company service will also make a difference.
> So, I encourage you to demo several different types of slide
> labelers and find the one that best suits your needs.
> Sharon Osborn
> DNAX
> ScheringPlough BioPharma
> Palo Alto, CA 
> 
> 
> Date: Tue, 10 May 2005 10:37:23 -0400
> From: "Favara, Cynthia (NIH/NIAID)" <cfavara <@t> niaid.nih.gov>
> Subject: [Histonet] slide labeller
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <F76C9B2DA2FC4C4CA0A18E288BBCBCF70A8DB35B <@t> nihexchange24.nih.gov>
> Content-Type: text/plain
> 
> I am interested in an automated slide labeler. I would like the capacity
> to
> do multiple slides with the same information as quiet as possible and of
> course I have limited space. Any suggestions would be appreciated.
> 
> 
> 
> I also have tops to the bottle for a Ventana special stainer. I am happy
> to
> send to anyone interested.
> 
> 
> 
> c
> 
> 
> 
> Cynthia Favara 
> NIAID/NIH/RML/LPVD
> 903 South 4th Street
> Hamilton, MT 59840
> 406-363-9317 
> 
> 
> 
> *********************************************************************
> This message and any attachments are solely for the intended recipient.
> If you are not the intended recipient, disclosure, copying, use or
> distribution of the information included in this message is prohibited
> -- Please immediately and permanently delete.
> 
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Tue, 10 May 2005 11:25:43 -0600
> From: Gayle Callis <gcallis <@t> montana.edu>
> Subject: Re: [Histonet] Asbestos studies
> To: mtitford <@t> aol.com, Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20050510112352.01b41688 <@t> gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
> 
> If I remember correctly, an iron stain will identify asbestos fibers
> nicely 
> in a section.  Histonet had discussion on asbestos fibers some time ago,
> 
> may be worth a search in archives at www.histosearch.org
> 
> At 09:52 AM 5/10/2005, you wrote:
>> Fred Underwood asks about asbestos fibers in lung tissues.
>> 
>> Asbestosis and mesothelioma studies are quite big business along the
> Gulf 
>> Coast here with the local shipyards and men of the "Golden Generation"
>> passing away. A lot of lawyers have made a lot of money and most
> recently, 
>> a big lawsuit in Corpus Christi Texas accuses screening companies of
>> misdiagnosis.
>> However, onto things histological: asbestos fibers can be easily seen
> in 
>> an H&E section as brown, cylindrical, and beaded. In our laboratory we
>> digest lung tissue in bleach and then filter the remains through a
>> millipore. Once dried, it can be placed on a slide, the filter
> dissolved 
>> in chloroform and mounted with a coverslip. The fibers are then
> counted.
>> The reference is Roggli VL, Oury, TD and Sporn TA " Pathology of
>> asbestos-associated diseases" 2ed edition Spinger. 2004
>> 
>> Mike Titford
>> USA Pathology
>> Mobile AL USA
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Tue, 10 May 2005 11:28:28 -0600
> From: Gayle Callis <gcallis <@t> montana.edu>
> Subject: [Histonet] Control for Mast cells
> To: "Anna Inman" <Anna.Inman <@t> stmarygj.org>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20050510112558.01b62930 <@t> gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
> 
> Skin contains mast cells in connective tissues and have seen them in
> connective tissues surrounding bones.
> 
> At 10:15 AM 5/10/2005, you wrote:
>> -->
>> 
>>  I am having difficulty getting a control for Mast cells. Any
> suggestions?
>> 
>> 
>> 
>> Anna Inman B.S., HT (ASCP)
>> 
>> SMH Pathology
>> 
>> <mailto:Anna.Inman <@t> stmarygj.org>Anna.Inman <@t> stmarygj.org
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
> Callis
>> Sent: Tuesday, May 10, 2005 8:51 AM
>> To: histosci <@t> shentel.net; Histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] Giemsa Stain for Mast Cells
>> 
>> 
>> 
>> Carsons pH 4.3 toluidine blue stain is excellent for Mast cells as is
>> 
>> Churukian Shenk method for mast cells.  You don't have to worry about
> so
>> 
>> many other colors from Giemsa.  If you want these, I will be happy to
>> 
>> attach privately.
>> 
>> 
>> 
>> At 07:45 AM 5/10/2005, you wrote:
>> 
>>> Netters,
>> 
>>> 
>> 
>>> 
>> 
>>> 
>> 
>>> I am looking for a Giemsa stain for Mast Cells that does not have a
>> 
>>> pinkish hue like the Gaffney Giemsa.  We are looking to have the Mast
>> 
>>> Cells pop out at us on low power.  Any suggestions/protocols?
>> 
>>> 
>> 
>>> 
>> 
>>> 
>> 
>>> Thanks,
>> 
>>> 
>> 
>>> 
>> 
>>> 
>> 
>>> Tom
>> 
>>> 
>> 
>>> 
>> 
>>> 
>> 
>>> Tom Galati
>> 
>>> 
>> 
>>> Laboratory Director
>> 
>>> 
>> 
>>> HSRL, Inc.- A GLP Compliant Contract Laboratory
>> 
>>> 
>> 
>>> 137 South Main Street
>> 
>>> 
>> 
>>> Woodstock, Virginia  22664
>> 
>>> 
>> 
>>> (540)459-8211
>> 
>>> 
>> 
>>> Fax: (540)459-8217
>> 
>>> 
>> 
>>> tomgalati <@t> hsrl.org
>> 
>>> 
>> 
>>> www.hsrl.org
>> 
>>> 
>> 
>>> 
>> 
>>> 
>> 
>>> _______________________________________________
>> 
>>> Histonet mailing list
>> 
>>> Histonet <@t> lists.utsouthwestern.edu
>> 
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> 
>> 
>> Gayle Callis
>> 
>> MT,HT,HTL(ASCP)
>> 
>> Research Histopathology Supervisor
>> 
>> Veterinary Molecular Biology
>> 
>> Montana State University - Bozeman
>> 
>> PO Box 173610
>> 
>> Bozeman MT 59717-3610
>> 
>> 406 994-6367 (lab with voice mail)
>> 
>> 406 994-4303 (FAX)
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> _______________________________________________
>> 
>> Histonet mailing list
>> 
>> Histonet <@t> lists.utsouthwestern.edu
>> 
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> This electronic message and all contents contain information from St.
>> Mary's Hospital  which may be attorney-client privileged, confidential
> or 
>> otherwise protected from disclosure.  The information is intended to be
> 
>> for the addressee only.  If you are not the addressee, any disclosure,
>> copy, distribution or use of the contents of this message is
>> prohibited.  If you have received this electronic message in error,
> please 
>> notify the sender immediately and destroy the original message and all
>> copies.  Thank you
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Tue, 10 May 2005 13:30:54 -0400
> From: "Jeffrey Wu" <wurochi <@t> hotmail.com>
> Subject: [Histonet] Picrosirius red stain
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BAY101-F3227F47CA5C3582A0340E0B21F0 <@t> phx.gbl>
> Content-Type: text/plain; format="flowed"
> 
> 
>  Hello all,
> 
>  I  am  trying  to  quantitate  the amount of fibrosis in mouse
> cardiac
>  tissue.   I  based  my  method  on  Dr Kiernan's excellent tutorial
> on
>  picrosirius  red  staining  (posted  in  2000).   Briefly,  slides
> are
>  deparaffinated  in  xylene  (2x, 6 min); 100% ethanol (2x, 3 min);
> 75%
>  ethanol  (1x,  3 min); and running distilled water (10-15 min).
> Next,
>  they  are stained  in  saturated  picrosirius  red  for 60 minutes
> and
>  washed in acetic acid (2x, 3 min).  They are dehydrated in 75%
> ethanol
>  (1x,  3  min);  100%  ethanol  (2x,  3  min);  and xylene (1x, 3
> min).
>  Finally, the slides are mounted with Permount.
> 
>  For  image  processing,  I am using circularly polarized light.
> Under
>  the  microscope,  the  collagen  stains  from pink to dark red
> (almost
>  black),  depending  on  its  content;  however,  the background
> tissue
>  appears  green/blue.    It  is  problematic  because  I  quantify
> the
>  collagen  using  ImagePro  Plus  software,  in  which I select
> certain
>  colors.  Especially  with  the  pink  and  some  dark  blues, there
> is
>  overlap  between  the  collagen  and tissue, causing overestimation
> of
>  collagen.  (Sorry  for  the  long explanation.)   I  guess what  I
> am
>  seeking  is the  flaw in my procedure, causing the other tissue not
> to
>  stain yellow.
> 
>  On  a side note, I am using the correct picrosirius red stain, and
> the
>  solution   is   saturated   with  crystals  at  the  bottom.   I
> have
>  tried modifying  washing  times without any change.  I used a short
> (2
>  min)  0.2%  phosphomolybdic  acid  wash,  which  is supposed to
> reduce
>  background, with no success as well.
> 
>  Once  again,  sorry  for  the long question.  Any help with my
> problem
>  would be greatly appreciated.  Please do not hesitate to email me
> with
>  any  questions  or  anything that I have not made clear.  Thank you
> in
>  advance.
> 
>  J Wu
> 
> 
> ------------------------------
> 
> Message: 6
> Date: Tue, 10 May 2005 13:52:27 -0400
> From: "Jim Staruk" <jstaruk <@t> masshistology.com>
> Subject: RE: [Histonet] Control for Mast cells
> To: "'Anna Inman'" <Anna.Inman <@t> stmarygj.org>,
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <0IGA002BACE3E8G4 <@t> vms048.mailsrvcs.net>
> Content-Type: text/plain; charset=us-ascii
> 
> Anyone in diagnostic veterinary services gets mast cell tumors all of
> the
> time.  I'll be glad to share some with someone in need (and I won't even
> charge you).
> 
> Jim
> 
> ______________________
>   Jim Staruk
> Mass Histology Service
> www.masshistology.com
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
> Callis
> Sent: Tuesday, May 10, 2005 1:28 PM
> To: Anna Inman; Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Control for Mast cells
> 
> Skin contains mast cells in connective tissues and have seen them in
> connective tissues surrounding bones.
> 
> At 10:15 AM 5/10/2005, you wrote:
>> -->
>> 
>>  I am having difficulty getting a control for Mast cells. Any
> suggestions?
>> 
>> 
>> 
>> Anna Inman B.S., HT (ASCP)
>> 
>> SMH Pathology
>> 
>> <mailto:Anna.Inman <@t> stmarygj.org>Anna.Inman <@t> stmarygj.org
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 7
> Date: Tue, 10 May 2005 13:58:36 -0400
> From: John Kiernan <jkiernan <@t> uwo.ca>
> Subject: Re: [Histonet] mast cell in guinea pig
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4280F64C.CD1F5085 <@t> uwo.ca>
> Content-Type: text/plain; charset=us-ascii
> 
> 
> 
> John Kiernan wrote:
>> 
>> Probably you didn't see any mast cells in the
>> sections of guinea-pig tissues because there
>> were none to be seen.
>> 
>> Mouse and rat mast cells are unusual in that their
>> granules are preserved and rendered insoluble by
>> aqueous fixatives such as buffered formaldehyde.
>> In most other species aqueous fixatives dissolve
>> out the mast cell granules. Alcoholic fixatives (eg
>> Carnoy, or the "alcoholic Bouin" fluids: Dubosq, Gendre
>> etc) will preserve and insolubilize mast cell granules
>> of any species.
>> 
>> For more on this, see "The Mast Cells" by Hans Selye
>> (1965) Chapter 3. Also the "Notes & Queries section in
>> the current issue of Biotechnic & Histochemistry (Vol 80
>> No 1, p.43-45). The latter is available at the publisher's
>> web site:
>> 
> http://journalsonline.tandf.co.uk/app/home/journal.asp?wasp=5a0cd1389c12
> 4b68b94584a9e888d3f3&referrer=parent&backto=searchpublicationsresults,1,
> 1;homemain,1,1;
>> 
>> The metachromasia is due to heparin, the major
>> macromolecular anion of the granules. The staining
>> properties of heparin are similar to those of
>> cartilage matrix. Both materials carry a lot
>> of sulphate-ester groups. Unfortunately heparin
>> (except that of rats and mice) is water-soluble.
>> I do not know if any tryptase or chymase immunoreactivity
>> remains in mast cells whose granules have been
>> extracted by an aqueous fixative.
>> --
>> -------------------------------
>> John A. Kiernan
>> Department of Anatomy and Cell Biology
>> The University of Western Ontario
>> London,   Canada   N6A 5C1
>>    kiernan[AT]uwo.ca
>>    http://publish.uwo.ca/~jkiernan/
>>    http://instruct.uwo.ca/anatomy/530/index.htm
>> _______________________________
>> Elizabeth Chlipala wrote:
>>> 
>>> Hello All
>>> 
>>> I'm looking for a stain that will identify mast cells in guinea
> pigs.  I
>>> have run both giemsa (modified dif quick) and 0.4% Toluidine Blue.
> I
>>> normally get very good staining of mast cells with the toluidine
> blue.
>>> I normally run this stain on mouse and rat tissue.  This is the
> first
>>> time I have tried this stain in guinea pigs.  Upon review of the
> slides
>>> I could not located one mast cell in 11 lung sections.  I know they
> have
>>> to be there.  Does the metachromatic nature of the t. blue stain
> have to
>>> do with the amount of histamine present in the cells?  I have read
> in
>>> the literature that guinea pigs and humans have less histamine
> present
>>> in their mast cells than rats, hamsters and mice.  Is anyone aware
> of a
>>> modification that will stain mast cells in guinea pig?  Any help
> would
>>> be appreciated.  I would prefer to stick with a histochemical
> method.
>>> I'm aware of Immunohistochemical staining for mast cell tryptase,
> but in
>>> researching this I could not find any references for guinea pig
> tissue.
>>> 
>>> Thanks in advance.
>>> 
>>> Liz
>>> 
>>> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>>> Manager
>>> Premier Laboratory, LLC
>>> P.O. Box 18592
>>> Boulder, Colorado 80308
>>> Office: (303) 735-5001
>>> Fax: (303) 735-3540
>>> liz <@t> premierlab.com
>>> www.premierlab.com
>>> 
>>> Ship to Address:
>>> Premier Laboratory
>>> University of Colorado
>>> MCDB, Room A3B40
>>> Boulder, Colorado 80309
>>> 
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet





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