[Histonet] antigen retrieval/enzyme digestion

[Patsy Ruegg]

I have heard of it being done both ways depending on the epitope of
interest.  In general I do HIER first and then EIER.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Till, Renee
Sent: Monday, May 02, 2005 5:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] antigen retrieval/enzyme digestion

When  trying both on the same slides, which would go first, the antigen
retrieval or the enzyme digestion?

 

Renee' Till, HT

 

Arkansas Childrens Nutrition Center

1120 Marshall

Little Rock, AR 

72202

 

(501) 364-2774

 


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