[Histonet] Mouse alveolar macrophages

[Corazon D. Bucana]

This antibody came from the lab of S. Gordon (the same lab that developed 
F4/80 antibody).  The antibody reacts to mouse macrophage scavenger 
receptor types I and II.  It stains tissue macrophages and is known to be 
present in abundance in atherosclerotic lesions.  My personal experience 
with this antibody is that macrophages found in wound healing reacts very 
strongly to this antibody and at a certain stage of wound healing it is 
possible to see macrophages stained by both antibodies but in the later 
stages of wound healing (eg. 10 days),F4/80 reactivity becomes weak but the 
anti-scavenger receptor is still very strong.   Strong reaction is also 
seen in several tumors as well.  since alveolar macrophages are essentially 
scavengers it seems reasonable that a strong reaction will also be seen in 
these cells. I hope this helps.

At 09:57 AM 5/5/2005 -0400, you wrote:
>I am not familiar with this antibody can you give more information? Is it
>from Serotec?
>c
>
>Cynthia Favara
>NIAID/NIH/RML/LPVD
>903 South 4th Street
>Hamilton, MT 59840
>406-363-9317
>
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>-----Original Message-----
>From: Corazon D. Bucana [mailto:bucana <@t> audumla.mdacc.tmc.edu]
>Sent: Wednesday, May 04, 2005 8:15 PM
>To: Thomas Crowell
>Cc: histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] Mouse alveolar macrophages
>
>You might want to test anti-scavenger macrophage antibody.  It works on
>frozen sections very well but Serotec might be able to tell you if it works
>on paraffin sections.
>
>At 02:34 PM 5/4/2005 -0400, you wrote:
>
> >Hi,
> >
> >Can anyone suggest an antibody that labels mouse lung macrophages in FFPE
> >tissue?  As confirmed with Serotec, F4/80 labels weakly or not at all.
> >
> >Thanks
> >Tom Crowell
> >BiogenIdec
> >Cambridge, MA
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>
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