[Histonet] mycoplasma staining
Alicia Henn Ph.D.
ahenn <@t> vaccinex.com
Mon May 2 13:29:56 CDT 2005
I use a bisbenzimide stain for routine mycoplasma detection of cell
cultures. Lately, I've had problems with a bright blue haze covering
dimly-stained cells. I've tried changing every step of the stain, but still
get blue fog. I fix with methanol/acetone (I've tried formalin as well)
stain the cells in chamberslides or in solution (for non-adherent cells) for
30 minutes, wash, air-dry chamberslides, mount with anti-fade mounting
medium (I've tried several). I can visualize fluorescent beads with
exquisite detail, so my microscope is OK. Anyone had any experience with
this? I don't want to have to go back to PCR-based detection as that was a
Alicia Henn, Ph.D.
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