[Histonet] Ki67 - kidney

[Coskran, Timothy M]

Christian,

We've been using an anti-rat Ki-67, clone MIB5, from DakoCytomation with
good results on formalin fixed paraffin embedded tissues.  We use a standard
ABC detection and anti mouse rat adsorbed secondary, after pre-treatment
using pH citrate buffer in a pressure cooker.  For Ki-67 antibodies, we've
had better success using a pressure cooker rather than a steamer for antigen
retrieval.

If you have any questions let me know,

Tim Coskran
Pfizer



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