ploykasek <@t> phenopath.com
Thu Mar 31 11:55:22 CST 2005
We use a CD2 clone MT910 on formalin fixed paraffin embedded (FFPE)
tissue. It is a bit tricky. We use a long heat retrieval with 10mM citrate,
pH6.0. We microwave pressure cook with this antibody, having the slides
under pressure for 15 minutes. Also, the antibody requires a tyramide
amplification detection system for FFPE. We use the CSAII from
Hope this helps. Good luck with it.
Does anyone know of a CD2 that works in FFPE tissue?
> E Earle
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet