[Histonet] CD2

[Patti Loykasek]

Elizabeth,
    We use a CD2 clone MT910 on formalin fixed paraffin embedded (FFPE)
tissue. It is a bit tricky. We use a long heat retrieval with 10mM citrate,
pH6.0. We microwave pressure cook with this antibody, having the slides
under pressure for 15 minutes. Also, the antibody requires a tyramide
amplification detection system for FFPE. We use the CSAII from
Dakocytomation. 
Hope this helps. Good luck with it.

Patti Loykasek
PhenoPath Laboratories
Seattle, WA>





 Does anyone know of a CD2 that works in FFPE tissue?
> 
> Thanks
> 
> E Earle
> 
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