[Histonet] neutral stains for protozoa

Mildred Fail failm <@t> musc.edu
Thu Mar 31 11:15:30 CST 2005


I use an aqueous 1% light green to counterstain in an Alizarin Red S 

Rena Fail
>> Gary Radice <gradice <@t> richmond.edu> 03/31/05 09:47AM >>>
I have a colleague who is studying a calcified protozoan. She is 
specifically interested in calcification, and she is looking for stains 
she can use that are not acidic and that don't require acid 
differentiation steps, so that she doesn't inadvertently decalcify the 
specimens.

Any thoughts about nuclear or counterstains that might work in this 
situation?



Gary P. Radice			gradice <@t> richmond.edu 
Department of Biology	804-289-8107 (voice)
University of Richmond	804-289-8233 (FAX)
Richmond VA 23173	http://www.richmond.edu/~gradice 


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