[Histonet] Frozen Spleen w/bubbles

[Kristen Broomall]

I'm sure someone more knowledgeable will come along soon, but could it be
freezing artifact? How are you freezing the samples?

Kristen Broomall

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jo Dee
Fish
Sent: Wednesday, March 30, 2005 5:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Frozen Spleen w/bubbles


Hello histonetters,

I am cryosectioning unfixed fresh frozen spleens and when they are 
sectioned I can see a billion bubbles under the section.  Can anyone 
explain-

1.  What is happening?
2.  How can I avoid them?
3.  Is there a fixation procedure that can help?

The sections are falling off of the slide during staining.
Thank you for your help,
Jo Dee
********************************************************************** 
**********

Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-5766
Fax: (415) 355-0230
E-mail: jfish <@t> gladstone.ucsf.edu

Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA   94158

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