[Histonet] Frozen Spleen w/bubbles

[Jo Dee Fish]

Hello histonetters,

I am cryosectioning unfixed fresh frozen spleens and when they are 
sectioned I can see a billion bubbles under the section.  Can anyone 
explain-

1.  What is happening?
2.  How can I avoid them?
3.  Is there a fixation procedure that can help?

The sections are falling off of the slide during staining.
Thank you for your help,
Jo Dee
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Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-5766
Fax: (415) 355-0230
E-mail: jfish <@t> gladstone.ucsf.edu

Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA   94158