[Histonet] Immunofluorescence and background

[Jerry Lyons]

Hi Histonetters,
 
My project is based on breaking immune tolerance to the self-antigen 
flk-1 for tumour therapy.
I am now trying to detect antibodies to flk-1 in whole mouse serum by 
immunofluorescence. The Flk-1 receptor is expressed on transfected BHK 
cells and I incubate the mouse serum at dilutions of 1:50, 1:100, and 
1:200 with the cells overnight at 4 degrees celcius. There are positive 
cells but the background is really obscuring the whole picture.
 
I would very much appreciate if anyone felt they could offer 
suggestions on eliminating the background on the immunofluorescence.
 
Thanking you in advance.
 
Jerry Lyons