[Histonet] Pneumocystis carnii staining

[anita dudley]

karen, in our lab we have found that we have to over stain the gms to get 
the pneumo to demonstrate.  hope this helps.  anita dudley, providence hosp. 
  mobile alabama

>From: "Karen Harmon" <karenharmon <@t> comcast.net>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Subject: [Histonet] Pneumocystis carnii staining
>Date: Mon, 28 Mar 2005 16:25:33 -0600
>
>We use a GMS stain to demonstrate pneuomcyctis with successful results 
>about 50% of the time.   We have taken this procedure apart step by step, 
>solution by solution and are at our wits end to determine the cause for 
>failure.  I can assure you that over the past 3 weeks, there is no variable 
>that we have not tried.    The ironic part is we never have a problem  
>demonstrating fungi with the GMS, in fact, we never had any problems at all 
>with other silver procedures.
>
>Can anyone help with our hit and miss problem with pneumocystis?
>
>Karen Harmon
>HT (ASCP)
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