[Histonet] Pneumocystis carnii staining
azdudley <@t> hotmail.com
Tue Mar 29 13:16:00 CST 2005
karen, in our lab we have found that we have to over stain the gms to get
the pneumo to demonstrate. hope this helps. anita dudley, providence hosp.
>From: "Karen Harmon" <karenharmon <@t> comcast.net>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Subject: [Histonet] Pneumocystis carnii staining
>Date: Mon, 28 Mar 2005 16:25:33 -0600
>We use a GMS stain to demonstrate pneuomcyctis with successful results
>about 50% of the time. We have taken this procedure apart step by step,
>solution by solution and are at our wits end to determine the cause for
>failure. I can assure you that over the past 3 weeks, there is no variable
>that we have not tried. The ironic part is we never have a problem
>demonstrating fungi with the GMS, in fact, we never had any problems at all
>with other silver procedures.
>Can anyone help with our hit and miss problem with pneumocystis?
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