[Histonet] Pneumocystis carnii staining

[Bonner, Janet]

 We had this problem until we stopped using Periodic Acid instead of Chromic
Acid.  Use the 4% Chromic Acid at 60 degrees C for an hour (we use a deep
waterbath) -or with a microwave and let sit for 20 minutes.
  We also had a problem with our control not being good enough (too old, I
guess).  We now purchase our controls from 'Newcomer' and they are
excellent.   
                            Janet

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Sent: 3/28/2005 5:25 PM
Subject: [Histonet] Pneumocystis carnii staining

We use a GMS stain to demonstrate pneuomcyctis with successful results
about 50% of the time.   We have taken this procedure apart step by
step, solution by solution and are at our wits end to determine the
cause for failure.  I can assure you that over the past 3 weeks, there
is no variable that we have not tried.    The ironic part is we never
have a problem  demonstrating fungi with the GMS, in fact, we never had
any problems at all with other silver procedures.

Can anyone help with our hit and miss problem with pneumocystis?

Karen Harmon
HT (ASCP)
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