[Histonet] Sliding vs. Sledge Microtome[Scanned]

[Kemlo Rogerson]

Oddly, in the UK, and I may be wrong on this, but......

There is a North/ South divide. From my experience the South tends to use
sledges (sliding microtomes) whilst the North use rotaries (except for the
hard stuff that needs a sledge). From my experience there is a tendency for
ribbon sections to be 'thick' and 'thin' (alternate ones that is) when cut
on a sledge and it has been suggested that rotaries can lead to 'chatters'.
>From my own experience I would not choose a sledge for biopsies, especially
if ribbons or levels are to be cut.

Why there is a 'North'/ South' divide eludes, maybe we Northerners eat more
bacon. Have you ever seen bacon cut on a sledge!!!!!

-----Original Message-----
From: Gudrun Lang [mailto:gu.lang <@t> gmx.at] 
Sent: 29 March 2005 12:42
To: Histonetliste (Histonetliste)
Subject: AW: [Histonet] Sliding vs. Sledge Microtome[Scanned]

We use sliding microtomes (Fa. Microm) for our routine histology, like most
of you probably use the rotary microtomes. The bigger sledge microtomes, I
consider, are mostly used with resinembeddings of hard material. 

Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Charles
Scouten
Gesendet: Montag, 28. März 2005 19:49
An: Traczyk7 <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] Sliding vs. Sledge Microtome

Your definition conforms to what I have seen, that sliding means moving
blade, and sledge means moving tissue.  However, I do not think there is any
functional difference.  They can be used for all the same applications, are
both grouped under the category "sliding microtome".  Does anybody in
histoland who has used both feel they have different applications?  There is
a clear safety difference, but the tissue doesn't care which moves.


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Traczyk7 <@t> aol.com
Sent: Friday, March 25, 2005 5:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sliding vs. Sledge Microtome

Help me out here. 
What is the generally accepted distinction between a sliding and a sledge  
microtome?  It seems to me that the terms get used interchangeably.   In my 
book, a "sliding" microtome has a fixed specimen holder and the knife
slides back and forth on a slide way.  It is used for routine or frozen
sectioning of samples.  A "sledge" microtome has a fixed blade and the
specimen is moved, either manually or motorized.  The general application
is for hard samples such as bone and in some material science  applications.

Any comments can be sent to me directly if you would like.
Thanks,
Dorothy Murphy Traczyk
Hacker Instruments & Industries Inc.
PO Box 1176
Winnsboro, SC 29180
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