[Histonet] fixatives

[Greer, Patricia]

Megan,
Here is the "recipe" for the JB fixative which we have used for fixation
of mouse tissues in which we wanted to detect CD3, CD4 and CD8.

JB Fixative

0.1 M  Tris Buffer, pH 7.4		1000ml
Calcium acetate			      0.5 g
Zinc Acetate				5.0 g
Zinc Chloride				5.0 g

Mix to dissolve.  The final pH will be approximately 6.8 (6.5-7.0).  Do
not readjust the pH, as this will cause the zinc to come out of
solution.  Small amounts of insoluable zinc oxychloride often
contaminates zinc chloride.  This may result in a very small amount of
white precipitate.  This will not cause any problems but can be removed
by filtering the solution.

Fix tissue for 24-48 hours, then transfer tissues to 70% ethanol.

Reference:
Beckstead JH. A Simple Technique for Preservation of Fixative-sensitive
Antigens in Paraffin-embedded Tissues: Addendum (Letter to the Editor).
J Histochem Cytochem 1994; 43:345


Pat Greer
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS G-32
Atlanta, GA 30333


I require some information regarding these fixatives. If anyone out in
Histonetland is is using any of these fixatives or has a reference for
me , I would be very happy and thankful.

CBA formalin
JB fixative
Excell

Thank you for your help in advance with my query.

HAPPY EASTER and HAPPY HOLIDAYS

Zenobia Haffajee
Hunter Area New England Health
IHC dept
Newcastle
Australia

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